Author(s): Nivetha. A, Mohanasrinivasan

Email(s): v.mohan@vit.ac.in

DOI: 10.5958/0974-360X.2018.00330.X   

Address: Nivetha. A, Mohanasrinivasan*
School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu
*Corresponding Author

Published In:   Volume - 11,      Issue - 5,     Year - 2018


ABSTRACT:
Lactose intolerance in humans is the major concern in current scenario. ß-Galactosidase has tremendous potential in the preparations of lactose free product development. The present research was carried out to screen ß-GalactosidaseproducingLactic acid bacteria (LAB) from milk and milk products such asdonkey milk, Curd and yogurt. Based on the morphological characteristics, about 70 bacterial colonies were selected. Out of these, 5 isolates from donkey milk, 7 from curd and 3 from yogurt sample were found positive for ß-Galactosidase production using X-Gal method. Potent strains were identified by determining the enzyme activity using ONPG assay.The maximum enzyme activity was found in isolate no. VITDM15 (Donkey milk) with of 375.21 AU/ml followed by 235.36 AU/ml from VITCD01(Curd) and 142.35 AU/ml from VITYT13(Yogurt) respectively. Hence these strains can be exploited for lactose free product development.


Cite this article:
Nivetha. A, Mohanasrinivasan. Screening and characterization of β-Galactosidase producing Lactobacillus sp. isolated from dairy samples. Research J. Pharm. and Tech 2018; 11(5):1778-1783. doi: 10.5958/0974-360X.2018.00330.X

Cite(Electronic):
Nivetha. A, Mohanasrinivasan. Screening and characterization of β-Galactosidase producing Lactobacillus sp. isolated from dairy samples. Research J. Pharm. and Tech 2018; 11(5):1778-1783. doi: 10.5958/0974-360X.2018.00330.X   Available on: https://rjptonline.org/AbstractView.aspx?PID=2018-11-5-14


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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