Author(s): Karrar Saleem Zayed

Email(s): karrars.alshebly@uokufa ,

DOI: 10.5958/0974-360X.2018.00195.6   

Address: Karrar Saleem Zayed
Department of Laboratory Investigations, Faculty of Science, University of Kufa, Iraq
*Corresponding Author

Published In:   Volume - 11,      Issue - 3,     Year - 2018

KRAS gene mutations in codon 12 are detected remarkably in high occurrence in Iraqi patients with colorectal adenocarcinoma. This study aims to investigate the mutations in codon 12 of this gene in colorectal tumor specimens. Fresh biopsies samples were collected from one hundred and twenty colorectal cancer (CRC)patients and one hundred (ulcerative colitis) as non-malignant negative control for revealing mutations in KRAS (codon 12) using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR), mutations were compared between patients and negative control, clinicopathologic features (age, gender, tumor size, grade, stage and tumor site) were compared between mutated and wild-type KRAS in CRC patients. Heterozygous mutations in KRAS codon 12 were significant only in individuals with CRC 52 of 120 (43.33%) when compared with non-malignant control group (ulcerative colitis) 0 (0%) (P<0.001), Odd ratio was 1.765; 95% CI was (1.765-2.202), other CRC patients were present in wild-type homozygotes 68(56.66%). High mean age, well-moderate grade, stage II and recto-sigmoid tumor was significant in association with heterozygous KRAS mutation in codon 12 than homozygous wild-type of CRC patients (P<0.05). Mutation in codon 12 KRAS is regarded as aprognostic marker used for early diagnosis of CRC, also it determines which patient responds to anti-EGFR targeted antibodies treatment.

Cite this article:
Karrar Saleem Zayed. Detection of Mutation in codon 12 of KRAS gene in Iraqi patients with colorectal Adenocarcinoma. Research J. Pharm. and Tech. 2018; 11(3): 1041-1047. doi: 10.5958/0974-360X.2018.00195.6

Recomonded Articles:

Author(s): Karpagam T, Jannathul Firdous, Revathy, Shanmuga Priya, Varalakshmi B, Gomathi S, Geetha S, Noorzaid Muhamad

DOI: 10.5958/0974-360X.2019.00360.3         Access: Open Access Read More

Author(s): B. Vijayakumar, Banurekha J, G. Swarnalatha, D. Jothieswari

DOI: 10.5958/0974-360X.2020.00387.X         Access: Closed Access Read More

Author(s): M. Krishna Moorthy, Bijaya Kumar Nayak, Anima Nanda, Anbarasi. P

DOI: 10.5958/0974-360X.2016.00071.8         Access: Open Access Read More

Author(s): Trilochan Satapathy, Prasanna Kumar Panda

DOI: Not Available         Access: Open Access Read More

Author(s): Chenmala Karthika, R. Sureshkumar, Dheepak Upadhyay, Janani S K, Vasanthi C, Raja M, Sai Surya Nikitha Upathyayula

DOI: 10.5958/0974-360X.2019.00563.8         Access: Open Access Read More

Author(s): Geetha Paramasivam, Sankaralingam Subbiah, Sugapriya Menaga Paulraj, Sivasankara Narayani Somasundaram, Jeyaraj Pandiarajan

DOI: 10.5958/0974-360X.2018.01001.6         Access: Open Access Read More

Author(s): ButhainahIbraheem Hameed Al-Ezzi, Ban Jumaah Qasim, Wijdan Basheer Abid, MuthannaIbraheem Al-Ezzi

DOI: 10.5958/0974-360X.2018.00139.7         Access: Open Access Read More

Author(s): Andrea Mary Francis, Ramya. R, Nalini Ganesan, Kumarasamy P, Solomon F. D Paul, Munirajan A. K, Roobini. J

DOI: 10.5958/0974-360X.2018.00840.5         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles