ABSTRACT:
The current aim of this work was to development and evaluation of sustain release pioglitazone microsphere. The microspheres were prepared by solvent evaporation method using polymers such as Ethyl cellulose, HPMC, Eudragit RL100. The prepared microspheres were characterized for particle size analysis, DSC, FTIR, XRD, DEE, in-vitro drug release and in vivo antidiabetic study. The in vitro drug release study suggested that the microspheres were capable of releasing drug up to 24 hours depending upon the formulation variables; The drug release was slow from the microspheres which were formulated with ethylene cellulose and eudragit RL100 as compared to those prepared with HPMC &Ethylene cellulose, HPMC & Eudragit RL100. The average particle size was in the range of 120-210 µm. Drug entrapment efficiency of pioglitazone loaded microspheres found in the range of 73.63% to 88.32%. The DSC analysis and X-ray diffraction study indicated that the drug uniformly dispersed in amorphous state in molecular level. The in-vivo anti-diabetic activity reveals that, the pure drug pioglitazone has shown maximal reduction in blood glucose at 2 hr and then reduction in blood glucose was decrease. But with the rat treated with p3 microsphere, the reduction in glucose level was initially slowly as compared to pure pioglitazone drug up to 2 hrs but it was slowly increased to 52.49% at 12 hr, suggesting the sustained and uniform release of pioglitazone over longer period from microsphere.