ABSTRACT:
Silver nanoparticles (AgNPs) was extracted by using clinical isolates for Staphylococcus aureus. The detection of AgNPsby qualitative method as Uv- visible. Antibacterial activity against (S .aureus, Pseudomonas, and Escherichia coli) isolates by plate diffusion method by using different concentrations (0.5, 0.25, 0.125, 0.062, 0.032) g/ml .the high significant differences among concentrations. Means of inhibition zones were higher with concentration at 0.5g/ml about (15.36 , 17.33and 13.5) mm while least inhibition zone with (0.065, 0.25 and 0.125) g/ml that appeared (11.27, 14.33 and 10.5) mm against S. aureus, Pseudomonas, and E. coli respectively. The AgNps mixed with some antibiotics and found that inhibition zone with mixed increased significantly than without silver nanoparticles, the conclusion from this study the AgNps was extracted from S.aureus and this nanoparticles had antibacterial activity against some bacteria and act synergistically with antibiotics