Author(s): Kamal Hossain, Kamrun Nahar, Mohammed Ehsanul Hoque Mazumder

Email(s): kamal@vetafarm.com.au

DOI: 10.5958/0974-360X.2017.00398.5   

Address: Md Kamal Hossain1*, Kamrun Nahar1, Mohammed Ehsanul Hoque Mazumder1, Md Wahiduzzaman1, Ali S. Alqahtani2, Dr Tony Gestier1, Kaiser Hamid1*
1Vetafarm Pty Ltd. R and D Centre, Wagga Wagga, NSW, 2650, Australia.
2Faculty of Pharmacy, King Saud University, Saudi Arabia
*corresponding author

Published In:   Volume - 10,      Issue - 7,     Year - 2017


ABSTRACT:
Development of a bioanalytical method is often tricky and labour intensive due to its nature or the criteria set by regulatory bodies. In the present study, a simple, reliable and sensitive analytical method was developed for the determination of levamisole residue in chicken eggs. Levamisole and ronidazole (internal standard, IS) were separated on a C18 reversed-phase HPLC column. Following liquid-liquid extraction, chromatographic separation was accomplished with a mobile phase consisting of acetonitrile: 0.05M KH2PO4 at ratio of 20:80, and the drug was detected at 235nm using a UV detector at a flow rate of 1.0ml/min at the ambient temperature. Linearity was obtained over the range 0.3125–10.0µg/ml for levamisole hydrochloride with lower limit of quantitation of 0.3125µg/ml. For each level of quality control samples, inter- and intra-day precision (%CV) was within ±15%. Absolutes extraction recovery of drug from plasma was =60%.The residue limit of levamisole gradually increased from day 1 to day 3 and then declined from day4 to day 7. The highest level of residue after oral administration of 80mg/kg levamisole to chickens was on day 3 (0.599µg/g). This level was lower than the recommended maximum residue limit (MRL), thus, no withholding period was required for egg consumption following the administration of the levamisole solution.


Cite this article:
Kamal Hossain, Kamrun Nahar, Mohammed Ehsanul Hoque Mazumder. Development and Validation of a Bioanalytical method for the Determination of Levamisole Residue in Backyard Poultry Egg. Research J. Pharm. and Tech. 2017; 10(7): 2249-2254. doi: 10.5958/0974-360X.2017.00398.5


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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