Author(s): K. Jagathy, K. Kalpana, S. Rajeshkumar

Email(s): ssrajeshkumar@hotmail.com

DOI: 10.5958/0974-360X.2017.00337.7   

Address: K. Jagathy1, K. Kalpana1, S. Rajeshkumar2
1Department of Microbiology, Sri Akilandeswari Women’s college, Wandiwash – 604 408, TN, India
2School of Bio-Sciences and Technology (SBST), Vellore Institute of Technology University, Vellore – 632014, TN, India
*Corresponding Author

Published In:   Volume - 10,      Issue - 6,     Year - 2017


ABSTRACT:
Aspergillus sp was isolated from soil and screened for glucose oxidase production. In this present investigation isolates 7 fungal isolates from different soil samples has shown positive glucose oxidase activity. The various parameters like Carbon Source (g/l) (glucose, sucrose, fructose, maltose) nitrogen Source (g/l) (peptone, yeast extract, beef extract), calcium carbonate (g/mL), pH, Temperature (ºC), incubation time and agitation rate used for the maximum enzyme production. The highest GOx production was obtained in glucose (100 g/L) followed by sucrose. Peptone (3-4 g/L) was found to be effective nitrogen source for GOx production when compared to other nitrogen sources. CaCO3 – 40 g/mL, pH – 6 and temperature 35°C and and incubation time 70 h for growth and production of glucose oxidase from screened culture of Aspergillus sps.


Cite this article:
K. Jagathy, K. Kalpana, S. Rajeshkumar. Production, optimization, Characterization and Immobilization of Glucose oxidase from Aspergillus species. Research J. Pharm. and Tech. 2017; 10(6): 1924-1928. doi: 10.5958/0974-360X.2017.00337.7


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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