Author(s): Veneta Simon, Sreerag K V, R Sasikumar, S K Kanthlal

Email(s): skkanthlal@aims.amrita.edu

DOI: 10.5958/0974-360X.2017.00307.9   

Address: Veneta Simon, Sreerag K V, R Sasikumar, S K Kanthlal*
Dept. of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, Amrita University, AIMS Health Science Campus, Kochi, Kerala, India
*Corresponding Author

Published In:   Volume - 10,      Issue - 6,     Year - 2017


ABSTRACT:
Aim and Objective: The aim of the study was to assess the degree of liver toxicity produced by commonly used antibiotics using in vitro liver slices. Method: The freshly isolated liver lobes were transferred into KRH medium and sliced into small pieces using surgical blade. It was then preincubated in KRH medium at 37°C by replacing fresh medium every 10 minutes for 1 hour. Then the slices were incubated with different concentrations of sulfamethoxazole, clavulanic acid and tetracycline for 2 hours. After 2 hours the prepared liver homogenate was assayed for the markers like ALT, AST, and MDA level and catalase activity for the assessment of toxicity. Results: After incubation with the three antibiotics at different doses, the liver parameters were altered in a dose dependent fashion. At the highest concentration of the antibiotics (2000 µg/ml) the parameters were found to be elevated which shows a clear indication of the degree of toxicity. Conclusion: The observed data reveals that repeated dosing and consuming these antibiotics at higher doses may lead to hepatotoxicity and it can be indicated by change in the parameters.


Cite this article:
Veneta Simon, Sreerag K V, R Sasikumar, S K Kanthlal. High dose antibiotics induced hepatotoxicity and altered markers: An in-vitro liver slices study. Research J. Pharm. and Tech. 2017; 10(6): 1742-1745. doi: 10.5958/0974-360X.2017.00307.9


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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