Author(s): Puja Kumari, Shatabdi Sen, Suneetha V

Email(s): vsuneetha@vit.ac.in

DOI: Not Available

Address: Puja Kumari, Shatabdi Sen, Suneetha V*
Department of Biotechnology, VIT University, Vellore-632014, Tamil Nadu, India
*Corresponding Author

Published In:   Volume - 10,      Issue - 6,     Year - 2017


ABSTRACT:
A key enzyme tannase enables the degradation of gallotannins, a type of hydrolysable tannins. A wide variety of microorganisms such as yeasts, fungi and bacteria leads to the production of tannase. Among these the tannase producing bacteria were commonly reported in dissipated water from paper, leather and forestry industries. In this study we will discuss about diverse bacteria that were isolated from the tannery effluent of several industries and its screening, characterization and the production of extracellular tannase. The production of extracellular tannase is highly influenced by Media Optimization and hence wide range of pH and temperature is utilized for the production of tannase and the optimal activity was found to be at 45°C and at a pH 6.5. The placket-Burman Logistic, Kinectic and Box-Behnken models were employed to optimize the media for tannase producing bacteria. In submerged culture (SMC) and solid-state (SSC) the induction and repression pattern of tannase production can be observed and the results presented that SSC has higher potential to minimize catabolite repression. Assessment of the effect of various additives on tannase activity was also done and thus among the various metal salts tested, the enzyme was found to be strongly inhibited byHgCl2 followed by ZnCl2 and MnCl2. Again it was also found that EDTA and ?- mercaptoethanol had inhibitory effect where as the detergents (Tween-20, -60 and -80 as well as SDS) did not affect the activity of the enzyme1. The recovery of glue and gelatin from tannery effluent was also studied and for that a three step hydrolysis process is best suited for maximum recovery of collagen hydrolysate followed by the glue and gelatine production from it. It was also reported in accordance with the antibiotic sensitivity test (Abst) that the bacteria were very sensitive for streptomycin.


Cite this article:
Puja Kumari, Shatabdi Sen, Suneetha V. Kinetic, Logistic, Box-Behnken model and statistical procedure for media optimization of tannase producing bacteria from Tannery Effluent and its application in Glue and gelatine production for pharma applications. Research J. Pharm. and Tech. 2017; 10(6): 1717-1725.


Recomonded Articles:

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Jino Elsa Thomas, Usha Y Nayak*, Jagadish PC, Koteshwara KB

DOI: 10.5958/0974-360X.2017.00007.5         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Siripurapu Sriram, Venkata Ravibabu Mandla, Chaithanya Sudha M, Saladi S.V. Subba Rao, Nagaveni Ch, Vani V., Gobinath P., Raj Kumar KVG., Jaydeep Lella, Sannidhi Krishna Praveen, Debjit Datta

DOI: 10.5958/0974-360X.2017.00078.6         Access: Open Access Read More

Author(s): Malarkodi Velraj, P. Jasmine Shiney, Biplab Paul, Rashmi. S, Nivethitha

DOI: 10.5958/0974-360X.2017.00006.3         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): N. Chandarsekaran, M. Balamurugan

DOI: Not Available         Access: Open Access Read More

Author(s): Barokar A.A., Wagh R.D., Baviskar D.T., Shaikh T.J.

DOI: Not Available         Access: Open Access Read More

Author(s): Krishnendu Acharya*, Somanjana Khatua, Salman Sahid

DOI: Not Available         Access: Open Access Read More

Author(s): Sajid Ali, Maryam Shabbir, Nabeel Shahid

DOI: 10.5958/0974-360X.2015.00019.0         Access: Open Access Read More

Author(s): Pooja Thakur, Chirag Chopra, Daljeet Singh Dhanjal, Reena Singh Chopra

DOI: 10.5958/0974-360X.2017.00466.8         Access: Open Access Read More

Author(s): Deependra Jain, Nishiprakash Jain, SK Jain, Nitin Jonwal, Shashank Mishra, Deepak Bhatt, Soham Shukla

DOI: Not Available         Access: Open Access Read More

Author(s): Kuldeep Kumar, Neelam Verma

DOI: Not Available         Access: Open Access Read More

Author(s): Arvindganth. R, Anupriya K.V., Kathiravan. G

DOI: 10.5958/0974-360X.2017.00105.6         Access: Open Access Read More

Author(s): Adithya Chandrashekar, Anuraag Muralidharan, Ananthamurthy Koteshwara, Angel Treasa Alex, V. M. Subrahmanyam

DOI: 10.5958/0974-360X.2019.00791.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags