Author(s): K.V Bhaskara Rao, Veena S, Pooja S, Shriya Y, Shivram R

Email(s): kokatibhaskar@yahoo.co.in

DOI: 10.5958/0974-360X.2017.00259.1   

Address: K.V Bhaskara Rao1*, Veena S2, Pooja S2, Shriya Y2, Shivram R2
1Professor, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore, TN - 632 014, India
2Molecular and Microbiology Research Laboratory, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India
*Corresponding Author

Published In:   Volume - 10,      Issue - 5,     Year - 2017


ABSTRACT:
Research for the selection of different enzyme producing microorganisms are increased around the world. Marine actinomycetes are one of the most investigated group since they are considered as a potential source of biotechnologically interesting products. The aim of the present study was to isolate commercially useful enzymes producing marine actinobacteria from Kanyakumari marine sediments. A total of 10 actinobacterial colonies were isolated which were designated as PV-1 to PV-10. In primary screening PV1, PV5, PV7 and PV8 showed good activity for cellulase and lipase enzyme production with a zone of hydrolysis of 10 mm and above. The isolate PV7 showed maximum activity with a zone of hydrolysis of 20 mm and 19 mm for cellulase and lipase enzyme respectively. Among all isolates PV7 and PV10 showed higher protease enzyme production with a zone of hydrolysis 19 mm and 14 mm respectively. In secondary screening PV7 showed maximum activity of 18.65 U/ml for cellulase followed by 15.32 U/ml of protease and 11.34 U/ml of lipase activities. The potential isolate PV7 was identified as Actinomycetales bacterium-PV7 using molecular methods by 16S rRNA sequencing. This study concludes that marine actinobacterial can be considered as source for the production of industrial and pharmaceutical enzymes.


Cite this article:
K.V Bhaskara Rao, Veena S, Pooja S, Shriya Y, Shivram R. Novel Actinomycetales bacterium-PV7 isolated from Kanyakumari Marine Sediments: A Prospective Source for Industrial and Pharmaceutical Enzyme Production. Research J. Pharm. and Tech. 2017; 10(5): 1471-1476. doi: 10.5958/0974-360X.2017.00259.1


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DOI: 10.5958/0974-360X 

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