ABSTRACT:
Research for the selection of different enzyme producing microorganisms are increased around the world. Marine actinomycetes are one of the most investigated group since they are considered as a potential source of biotechnologically interesting products. The aim of the present study was to isolate commercially useful enzymes producing marine actinobacteria from Kanyakumari marine sediments. A total of 10 actinobacterial colonies were isolated which were designated as PV-1 to PV-10. In primary screening PV1, PV5, PV7 and PV8 showed good activity for cellulase and lipase enzyme production with a zone of hydrolysis of 10 mm and above. The isolate PV7 showed maximum activity with a zone of hydrolysis of 20 mm and 19 mm for cellulase and lipase enzyme respectively. Among all isolates PV7 and PV10 showed higher protease enzyme production with a zone of hydrolysis 19 mm and 14 mm respectively. In secondary screening PV7 showed maximum activity of 18.65 U/ml for cellulase followed by 15.32 U/ml of protease and 11.34 U/ml of lipase activities. The potential isolate PV7 was identified as Actinomycetales bacterium-PV7 using molecular methods by 16S rRNA sequencing. This study concludes that marine actinobacterial can be considered as source for the production of industrial and pharmaceutical enzymes.