Author(s): Jane Jacob, Sreekanth Nadig

Email(s): janempharm@gmail.com

DOI: 10.5958/0974-360X.2017.00823.X   

Address: Jane Jacob*, Sreekanth Nadig
Nitte University, Dept. of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences, Deralakatte, Mangalore -575018,Karnataka State.
*Corresponding Author

Published In:   Volume - 10,      Issue - 12,     Year - 2017


ABSTRACT:
A simple, fast, reproducible, reliable and sensitive UPLC method was developed for the simultaneous estimation of anti-retroviral drugs in combination namely, Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz. The chromatographic separation was carried out on a sub 2 micron particle size column Waters Acquity UPLC BEH C18, 100 x 2.1 mm, 1.7 µm to achieve more efficiency and better resolution in a gradient mode elution consisting of mobile phase A : Buffer (0.05% of Trifluro acetic acid in water) and mobile phase B: Methanol. Detection wavelength was set at 262 nm with a flow rate of 0.4ml/min. The retention times of Emtricitibine, Tenofovir disoproxil fumarate and Efavirenz was 0.6, 1.88 and 3.23 minute respectively. Under optimized conditions all three components were well separated from each other and also from main degradation impurities like Monoester impurity of Tenofovir, S-Oxide impurity of Emtricitabine and Amino alcohol and Quinoline impurity of Efavirenz. This was further supported by forced degradation studies. The % RSD was well within the limits and the correlation coefficient was 0.999.The developed method was validated as per ICH guidelines and can be used in routine quality testing of individual dosage forms and in combination.


Cite this article:
Jane Jacob, Sreekanth Nadig. A UPLC method for simultaneous estimation of emtricitabine, tenofovir disoproxil fumarate and efavirenz in pharmaceutical dosage forms. Research J. Pharm. and Tech 2017; 10(12): 4463-4466. doi: 10.5958/0974-360X.2017.00823.X


Recomonded Articles:

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): YK Naidu, Raghunadha Reddy S, Koteswara Rao Divi, MP Kalyan Reddy, I Sarath Chandiran, KN Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): S. Poongothai, C.M. Karrunakaran, R. Ilavarasan

DOI: Not Available         Access: Open Access Read More

Author(s): Oruganti Sai Koushik, V. Himaja, P. Srinivasa Babu, Ramadoss Karthikeyan

DOI: 10.5958/0974-360X.2015.00298.X         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags