Author(s): Phani R.S.Ch, K.R.S. Prasad, Useni Reddy Mallu

Email(s): phani.r.s.ch@gmail.com

DOI: 10.5958/0974-360X.2017.00781.8   

Address: Phani R.S.Ch1 , K.R.S. Prasad1, Useni Reddy Mallu2*
1Department of Chemistry, K.L. University, Vaddeswaram-522502, Andhra Pradesh, India.
2R. V. Labs, Guntur-522007, Andhra Pradesh, India.
*Corresponding Author

Published In:   Volume - 10,      Issue - 12,     Year - 2017


ABSTRACT:
New High-performance liquid chromatographic bio-analytical method has been developed and validated for the simultaneous analysis of Trifluridine (TFD) and Tipiracil (TPC) in human plasma. The combination of TFD and TPC are used in the treatment of unresectable advanced or recurrent colorectal cancer. The method was developed with UV detector (at 260 nm wave length), Intersil ODSC 18 column (250 mm × 4.6 mm×5µ), at flow rate of 1.0mL/min. The mobile phase consisted of 15% NaClO4 buffer (PH 4.5 v/v), 85% Methanol. The retention times of TFD and TPC are 3.4 min and 7.4 min respectively. The method was developed and validated in terms of selectivity, precision, linearity, accuracy, LOQ, LOD and stability study. The proposed method can be applicable for pharmacokinetic studies using HPLC or LC-MS.


Cite this article:
Phani R.S.Ch, K.R.S. Prasad, Useni Reddy Mallu. New Bio Analytical Method Development and Validation for the Simultaneous Estimation of Trifluridine and Tipiracil in spiked human plasma. Research J. Pharm. and Tech 2017; 10(12): 4264-4268. doi: 10.5958/0974-360X.2017.00781.8


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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