During the last years, the discovery of new anticancer drugs remains the mains concerns in oncology, The natural environment has always been a very important source of active Biological molecules and which 60% of the cancer drugs used currently are original from a natural products. H.albus is a plant which belongs to Solanaceae family, used generally in traditional medicine as a nervous sedative and para sympatholytic which is rich source of flavonoid, alkaloids and tropane. The present work is for anobjective to test the methanolic fractions extracted from the Aerial Parts of Hyoscyamus albus collected among aures region in Algeria, and evaluate their cytotoxic activity on different cancercells lines and the characterization of the microscopical morphology of apoptosis cell. The effects of four different selected methanolic fractions of H.albusextract( D, E, F, G)obtained by column chromatography over Sephadex LH-20 tested on DU-145, PC-3, U-87 MG and U-373 MG cancer cells lines were determined using MTT [3-(4,5-dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide] assay and the cell were marked by orange acridine to visualize the apoptotic cells. Our results indicated that the fraction C has a cytotoxic activity against cells lines showed a marked anti-cancer activity with IC50 = 73µg/ml, 187 µg/ml, 96 µg/ml and 114 µg/ml for the DU-145, PC-3, U-87 MG and U-373 MG respectively, the morphotype apoptotic cell have showed the morphological change which attempt an apoptotic cell marked by a fluorescence. Therefore, the fraction D had just an activity against DU-145 with IC50=186 µg/ml, the fraction F has an IC50 =165 µg/ml and 198 µg/ml against DU-145 and PC-3 respectively. The fraction G showed the activity against DU-145, LNCaP and U-373 MG. The results demonstrated the anticancer properties of H.albusand reveal the therapeutic potential of this plant.
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