ABSTRACT:
Antimicrobial confrontation has emerged as one of the grim difficulties posing great task to pharmaceutical and medical sectors at global level. This increase in drug resistant strains facilitates easy transfer of their resistant gene(s) through an ecosystem of susceptible bacteria resulting in the emergence of superbugs. Amongst resistance pattern displayed by multidrug resistant strains, enzymatic means of defense i.e., ESBL (Extended Spectrum ß-lactamases) remain one of the effective strategy towards broad spectrum antibiotics. These plasmid encoded ß-lactamases hydrolyse the oxyimino ß-lactam ring nullifying the antibacterial efficacy of most of the cell wall inhibitors. Despite hospital and institutional care infections, the members of Enterobacteriaceae (E. coli) with CTX-M gene accounts for most of the community-acquired urinary infections. In the present study, isolates of pathogenic ESBL producing E. coli causing UTI infections from hospitals were detected for their virulence gene using Polymerase chain reaction (PCR) and were investigated their antibiotic sensitivity pattern. Antibiotic sensitivity was recorded high for Carbapenems (Imipenem, Meropenem, Ertapenem and Imipenem- Cilastatin) (100%) followed by Ofloxacin (93.47%) and Doxycycline hydochloride (92.39%). Least sensitivity was recorded for Cephalosporins (Cefotaxime, Ceftriaxone, Ceftazidime (8.69%). Our current study supports the finding that UTI caused by Pathogenic ESBL producing E. coli in nosocomial infection is found to be high. This work further explains the need of strict monitoring and surveillance for effective measures of hygiene and sanitary practice within hospitals especially in intensive care units and different wards.