Author(s): Ilham A. Bunyan, Qasim Ali Jassem Alkhuzaee

Email(s): Ilhamalsaedi2008@gmail.com

DOI: 10.5958/0974-360X.2017.00584.4   

Address: Ilham A. Bunyan*, Qasim Ali Jassem Alkhuzaee
Dept. of Microbiology, College of Medicine, Babylon, Al – Qasim General Hospital, Babylon Health Directorate, Hilla, Iraq
*Corresponding Author

Published In:   Volume - 10,      Issue - 10,     Year - 2017


ABSTRACT:
In this study, (100) clinical samples were collected from patients with urinary tract infection (UTI). Samples were then subjected for culturing on selective media to isolate Enterobacter cloacae. All clinical samples were positive culture, only (62) samples showed positive cultures, as gram negative bacteria whereas (38) bacterial growth were detected as gram positive bacteria. Among (62)gram negative bacteria, only 12(19.4%) isolates belonged to Enterobacter cloacae, isolates were identified by API20E. Sequencing of hsp60 gene and alignment of (341) base pare confirmed the identity of 8(12.9%) isolates. All DNA samples extracted from bacterial isolates were conducted for polymerase chain reaction to investigate presence of curli genes among isolates. The result revealed that 8(100%) of isolates were positive to Curli-specific Genes D(CsgD)gene, The Curli-specific Genes D (csgD)genes showed a bands of expected size (355) base pare in (8) isolates E. cloacae under study. Six of eight Enterobacter cloacae isolates produced (245) base pare amplification bands of Curli-specific Genes A (csgA)gene (75%). The results display significant positive correlation between biofilm formation and curlifambriae adhesion. Our results conclude the importance of curli fimbriae adhesion in establishment of biofilm and magnitude of biofilm formation in urinary tract infection (UTI)via antibiotics resistance and ascending infections.


Cite this article:
Ilham A. Bunyan, Qasim Ali Jassem Alkhuzaee. Detection of Curlibiogenesis genes among Enterobacter cloacae isolates and their role in biofilm formation in Al-Hilla City, Iraq. Research J. Pharm. and Tech 2017; 10(10):3294-3300. doi: 10.5958/0974-360X.2017.00584.4


Recomonded Articles:

Author(s): Hisham A. Abbas, Mona A. El-Sayed, Laila M. Al-Kadi, Amany I. Gad

DOI: Not Available         Access: Open Access Read More

Author(s): Salwani Ismail, Noor Zarina Abd Wahab, Norhidayah Badya, Nor Iza A. Rahman, Chew Chieng Yeo, Ahmad Zubaidi A. Latif, Mainul Haque

DOI: Not Available         Access: Open Access Read More

Author(s): Daljeet Singh Dhanjal, Chirag Chopra, Prince Anand, Reena Singh Chopra

DOI: 10.5958/0974-360X.2017.00612.6         Access: Open Access Read More

Author(s): Samrithi Yuvaraj, Gheena. S

DOI: 10.5958/0974-360X.2015.00200.0         Access: Open Access Read More

Author(s): NasimunIslam N1., Jemimah Naine S., Shaik Jameel, S.S. Khora, Venkat Kumar, Mohanasrinivasan V., C. Subathra Devi, Panneerselvam A.

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags