ABSTRACT:
Soil is the richest ecosystem there is, as far as the diversity and populations of microorganisms is concerned. It is known that there could be over ten billion microbes per gram of soil, making soil the ideal ecosystem for mining different genes, ORFs etc. Microbial Diversity forms the lifeline of an ecosystem, interfering, regulating and participating in the biogeochemical activities therein. The present study was conducted to explore and assess the microbial community by culture dependent and independent approach, of sugarcane fields of Gujjarwal Village of Ludhiana. To study microbial diversity in sugarcane fields, a total of nine different colonies were isolated and differentiated based on their cell morphology and size. Biochemical characterizations lead to the identification of Enterobacter cloacae, Proteus mirabilis and Serratia marcescens. Molecular characterization was done by isolating soil DNA (GJSF DNA) and subjected to PCR amplification for identification via 16S rRNA gene analysis. BLAST analysis revealed presence of Serratia sp., uncultured bacterium clones SS-22C07, ep138, K-15-14-19, S127B31, L1A.11C01 and uncultured Enterobacteriaceae bacterium.