Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

Email(s): asksankar1@gmail.com

DOI: 10.5958/0974-360X.2017.00047.6   

Address: A. S. K. Sankar*, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani
Department of Pharmaceutical Chemistry and Analysis, School of pharmaceutical sciences, Vels University (VISTAS) , Chennai-600117
*Corresponding Author

Published In:   Volume - 10,      Issue - 1,     Year - 2017


ABSTRACT:
Aim: The aim is to develop simple validated analytical method for analysis of Tofacitinib by RP HPLC in pure and laboratory prepared physical admixtures. Method: Tofacitinib was estimated by RP HPLC using methanol , water mixture as mobile phase. Linearity range was found to be 10-50 mcg/ml. The correlation coefficient was 0.9998. The limit of detection and quantification were found to be 0.053 and 1.63 µg /ml respectively. The drug solutions were scanned in UV spectrophotometry and the maximum absorption was found to be 285.9 nm and the same was fixed as detecting lambda by RP HPLC method. The Column used was C18.with the dimensions of 150 mm X 4.6 mm and 5 micron particles. Results and Conclusions: The proposed method was successfully applied for the determination of tofacitinib in pure and laboratory prepared physical mixtures. The % RSD value of Tofacitinib in bulk and physical admixture was calculated at different time intervals for recovery , precision (Iintraday and Interday experiments) and quantification studies were found to be less than 2 %.


Cite this article:
A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani. Development of a Validated Reverse Phase Liquid Chromatographic Assay-Method for determination of Tofacitinib in pure form and in Physical Admixtures. Research J. Pharm. and Tech. 2017; 10(1): 223-226. doi: 10.5958/0974-360X.2017.00047.6


Recomonded Articles:

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Malarkodi Velraj, P. Jasmine Shiney, Biplab Paul, Rashmi. S, Nivethitha

DOI: 10.5958/0974-360X.2017.00006.3         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): YK Naidu, Raghunadha Reddy S, Koteswara Rao Divi, MP Kalyan Reddy, I Sarath Chandiran, KN Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): S. Poongothai, C.M. Karrunakaran, R. Ilavarasan

DOI: Not Available         Access: Open Access Read More

Author(s): Oruganti Sai Koushik, V. Himaja, P. Srinivasa Babu, Ramadoss Karthikeyan

DOI: 10.5958/0974-360X.2015.00298.X         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 


Recent Articles




Tags