Author(s): Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali

Email(s): khanhamid770@gmail.com

DOI: 10.5958/0974-360X.2016.00278.X   

Address: Hamid Khan1* Mushir Ali2, Alka Ahuja2, Javed Ali2
1JK College of Pharmacy, Bilaspur, CG, India-495001
2Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India-110062
*Corresponding Author

Published In:   Volume - 9,      Issue - 9,     Year - 2016


ABSTRACT:
In the presented work the ultra-performance liquid chromatographic/quadrupole time-of-flight mass spectrometric (UPLC/Q-TOF-MS) method has been developed for simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. For identification of drugs, the Q-TOF mass spectrometer was operated in negative ionization mode and quantification was done using the MS/MS transitions at m/z 513.18 to 469.13 for telmisartan, and 268.90 to 204.94 for hydrochlorothiazide. The chromatographic separation was achieved on Acquity UPLCTM BEH C18 (100.0 × 2.1 mm, 1.7µm) column using isocratic mobile phase consisting of acetonitrile-2mM ammonium acetate (50:50, v/v) at a flow rate of 0.25 mL/min. The elution of telmisartan and hydrochlorothiazide was occurred at 2.25 and 1.22 min, respectively. The calibration curves were linear over the concentration range of 1-1000 ng/mL for both the drugs. The developed method was validated according to ICH guidelines. The method was applied for pharmacokinetic study of telmisartan and hydrochlorothiazide in human plasma.


Cite this article:
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali. Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Human Plasma. Research J. Pharm. and Tech 2016; 9(9):1441-1446. doi: 10.5958/0974-360X.2016.00278.X

Cite(Electronic):
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali. Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Human Plasma. Research J. Pharm. and Tech 2016; 9(9):1441-1446. doi: 10.5958/0974-360X.2016.00278.X   Available on: https://rjptonline.org/AbstractView.aspx?PID=2016-9-9-30


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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