Author(s): Hajare Ashok A, Powar Trupti A, Bhatia Neela M., More Harinath N

Email(s): ashok.hajare@bharatividyapeeth.edu

DOI: 10.5958/0974-360X.2016.00259.6   

Address: Hajare Ashok A.*, Powar Trupti A., Bhatia Neela M., More Harinath N
Department of Pharmaceutical Technology, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur, Maharashtra, India -416013
*Corresponding Author

Published In:   Volume - 9,      Issue - 9,     Year - 2016


ABSTRACT:
A rapid reversed phase (RP) high performance liquid chromatography (HPLC) method was developed and validated for quantitative estimation of Doxorubicin hydrochloride (Dox) from the prepared vacuum foam dried formulation. Chromatographic runs were performed on C18 column with mobile phase comprising of acetonitrile: 0.01M o-phosphoric acid (40:60) pH 2 at a flow rate of 0.75mL.min-1. Dox was detected at a wavelength of 234nm. The method was shown to be specific, linear in the range of 0-10µg.mL-1 with correlation coefficient of 0.9999, precise at the intra-day level as reflected by relative standard deviation, accurate at recovery rate 99.60±0.13 and robust to change mobile phase and column brand. The detection and quantitation limits were 0.091µg.mL-1 and 0.2758µg.mL-1, respectively. The proposed method could be advantageous in estimation of Dox quantitation in pharmaceutical dosage form in the presence of excipients.


Cite this article:
Hajare Ashok A, Powar Trupti A, Bhatia Neela M., More Harinath N. Development and Validation of RP-HPLC Method for Determination of Doxorubicin Hydrochloride from Vacuum Foam Dried Formulation. Research J. Pharm. and Tech 2016; 9(9):1352-1356. doi: 10.5958/0974-360X.2016.00259.6

Cite(Electronic):
Hajare Ashok A, Powar Trupti A, Bhatia Neela M., More Harinath N. Development and Validation of RP-HPLC Method for Determination of Doxorubicin Hydrochloride from Vacuum Foam Dried Formulation. Research J. Pharm. and Tech 2016; 9(9):1352-1356. doi: 10.5958/0974-360X.2016.00259.6   Available on: https://rjptonline.org/AbstractView.aspx?PID=2016-9-9-11


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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