Sai Vijay M, D. Viswanath Guptha, Muddu Krishna B S, S G Vasantharaju
Sai Vijay M, D. Viswanath Guptha, Muddu Krishna B S, Dr. S G Vasantharaju*
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences,
Manipal University, Manipal- 576 104, Karnataka, India.
Volume - 9,
Issue - 8,
Year - 2016
Analysis was performed using Gracesmart C18 column (250 × 4.5mm; 5µm). Isocratic mode of separation was followed using mobile phase which contains acetonitrile and phosphate buffer (pH adjusted to 4.0 by using ortho phosphoric acid) in proportion of 20:80 v/v. The developed method employs use of UV detector with detection wavelength of 225 nm, volume of injection was 20 µl and employing flow rate of 1ml/min. The Rt of the drug under study was found to be 6.0 ± 0.3mins. Method was precise, accurate, reproducible, linear and robust. The method was linear between 0.1 µg/ml to 25 µg/ml with r2 of 0.9991. The method would be applicable for assay of tablet dosage from, stability and dissolution of marketed Naratriptan hydrochloride tablets. The % assay for tablets using the proposed method was found to be 100.17. The % RSD from system and method precision were 0.48 and 0.79 respectively. Accuracy was found to be 100.5%.
Cite this article:
Sai Vijay M, D. Viswanath Guptha, Muddu Krishna B S, S G Vasantharaju. Stability Indicating Assay Method Development and Validation of Naratriptan Hydrochloride By RP–HPLC. Research J. Pharm. and Tech 2016; 9(8):1177-1182. doi: 10.5958/0974-360X.2016.00225.0