Author(s): N.Tamilselvi, Hempushpa Sinha, Deepthi Visakh, P.Vanathi


DOI: 10.5958/0974-360X.2016.00126.8   

Address: N.Tamilselvi1*, Hempushpa Sinha1, Deepthi Visakh1, P.Vanathi2
1Department of Pharmaceutical Analysis, K.M.C.H College of Pharmacy, Kalapatti Road, Coimbatore-641048, Tamil Nadu, India
2Department of Pharmaceutical Chemistry, Karpagam College of Pharmacy, Coimbatore. Tamil Nadu, India
*Corresponding Author

Published In:   Volume - 9,      Issue - 6,     Year - 2016

A simple, reliable HPLC method was developed and validated for quantitation of clotrimazole in human plasma. Preparation was carried out using protein precipitation method. Separation was done on a Enable C18 reverse phase column with a mobile phase of 0.5% TEA in water (pH 3 adjusted with o-phosphoric acid) and acetonitrile in the ratio of 20:80%v/v and detection was done at 215nm. The retention time of ibuprofen (IS) and Clotrimazole was found to be 5.2 and 8.2mins respectively. The method was developed in terms of accuracy, precision, linearity, specificity, system suitability, and stability studies, limit of detection, limit of quantification, ruggedness and robustness. The calibration curve was linear within the range of 50-250ng/ml (r2>0.999) . The lowest limit of quantification and detection was found to be 23.90ng/ml and 7.8 ng/ml respectively. System suitability test results on the retention time, area ratio and peak ratios of peak chromatogram shows the CV (%) <10%. The proposed method was validated and proved to be adequate for the determination of Clotrimazole in human plasma.

Cite this article:
N.Tamilselvi, Hempushpa Sinha, Deepthi Visakh, P.Vanathi. Bio-analytical method development and validation for the estimation of Clotrimazole in human plasma by RP-HPLC method. Research J. Pharm. and Tech. 2016; 9(6): 671-676. doi: 10.5958/0974-360X.2016.00126.8

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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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