ABSTRACT:
An endophyte is an organism which lives in the interior tissues of the plants without causing any harmful effect to the host plants. These organism includes bacteria, actionomycetes and fungi. Endophytes are now extensively utilized in modern medicine and agriculture because of their distinct capacity of producing a wide range of metabolites with effective biological activities. In the present study, anti bacterial activity of endophytic fungal organism Phoma europhyrena isolated from leaf tissue of Clerodendron sp and the antibacterial activity was studied against human pathogenic bacteria Staphylococcus aureus and Pseudomonas aeruginosa. Surface leaf washing method was adopted to isolate the fungal organism and the isolated fungi was cultured in synthetic liquid media under optimum condition. Extracts were prepared from culture free broth using ethyl acetate. Concentrated crude extract thus obtained was tested against the bacterial strains by agar diffusion solid plate assay and turbidometric liquid broth assay. Antibacterial activity was further confirmed by biofilm inhibition studied by microtitre plate assay .The present findings clearly reveals tested bacterial strains were susceptible to ethyl acetate extract which were all studied by agar diffusion, turbidometric and biofilm inhibition assays. Further studies will helpful to identify the bioactive compound and the mechanism of action which would suggests the utilization of fungal extracts as an effective antibacterial agents.