ABSTRACT:
The chief goal of our current research was to establish the anticancer property of Colchicine, a fresh compound isolated from Indigofera aspalathoids. Hep3B cell lines were treated with Colchicine for 24 hrs, 48 hrs and 72 hrs. MTT assay was performed to check the degree of cytotoxicity. The morphological changes of colchicine treated cell lines and control groups were observed using phase contrast microscope. Acridine Orange/Ethidium Bromide (AO/EB) double staining, Propidium iodide (PI), were done to check apoptosis of Hep3B cell lines. Cell cycle analysis was employed to determine apoptosis in Hep3B cell lines and degree of apoptosis was quantified using flow cytometry. Colchicine induces cell death in Hep3B cell lines in a dose dependent manner. The IC50 (half maximal inhibitory concentration) value of colchicine on Hep3B cells was calculated as 344.25 µg/ml. Control group cell lines showed normal morphology, whereas the Colchicine treated cells showed altered morphology, also cells were not properly adhered to the culture flask. Colchicine induces apoptosis in Hep3B cell lines. Thus, it could be used as an active member of pharmaceutical or therapeutic concerns for the drug development.