Author(s): Venkataramana N.V., Nivedita R Desai, Sreenivasa S, Chaluvaraju K. C., Aruna Kumar D. B.

Email(s): nirmaldb@rediffmail.com

DOI: 10.5958/0974-360X.2016.00444.3   

Address: Venkataramana N.V.1, Nivedita R Desai1, Sreenivasa S1, Chaluvaraju K. C.2, Aruna Kumar D. B.*1
1Department of Studies and Research in Chemistry, Tumkur University, Tumakuru-572103, India
2Deptartment of Pharmaceutical Chemistry, Government College of Pharmacy, Bengaluru-560 027, India.
Corresponding Author

Published In:   Volume - 9,      Issue - 12,     Year - 2016


ABSTRACT:
In the present study arapid, simple, selective, linear, precise, accurate and robust reverse phase high performance liquid chromatography method was developed and validated for the qualitative and quantitative analysis of rifampicin in bulk and pharmaceutical formulations and their degradation studies such as acid, alkali and peroxide stressed were carried out. Gradient elution at a flow rate of 1.0 ml min-1 was employed using symmetry syncronis C18 (250 x 4.6 mm 5µm), column at a temperature of 400 C. The mobile phase used consisted of phosphate buffer (A) and acetonitrile (B) (100%) in the ratio of 50:50 v/v. The chromatograms were recorded at a wavelength of 236 nm using array detector. Linearity was observed in the concentration range of 25-125 µg/ml. The retention time of rifampicin was found to be 5.2 min. The method was validated as per the international conference on harmonization (ICH) guidelines and the proposed method can be successfully applied for the estimation of rifampicin in pharmaceutical formulations.


Cite this article:
Venkataramana N.V., Nivedita R Desai, Sreenivasa S, Chaluvaraju K. C., Aruna Kumar D. B. Development and Validation of a Chromatographic Method for the Estimation of Rifampicin in Bulk and Pharmaceutical Formulations. Research J. Pharm. and Tech 2016; 9(12):2191-2198. doi: 10.5958/0974-360X.2016.00444.3


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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