Author(s): Deblina D, Namrata M, Taishee P, Veena S, Bhaskara Rao K.V

Email(s): kokatibhaskar@yahoo.co.in

DOI: 10.5958/0974-360X.2016.00443.1   

Address: Deblina D, Namrata M, Taishee P, Veena S and Bhaskara Rao K.V.*
Molecular and Microbiology Research Laboratory, Department of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India
Corresponding Author

Published In:   Volume - 9,      Issue - 12,     Year - 2016


ABSTRACT:
Biosurfactants are the surface active molecules that are produced by microorganisms. The present study focused on the isolation of biosurfactant producing bacteria from oil contaminated soil samples of an automobile mechanical workshop near VIT University, Vellore. A total of 5 bacterial colonies were isolated from the collected samples (DNT-1 to DNT-5). Among them the isolate DNT-2 showed maximum biosurfactant production followed by DNT-3 and DNT-5. In primary screening DNT-2 showed a good haemolytic activity with a zone of clearance of 18 mm followed by DNT-3 (12 mm) and DNT-5 (10 mm). All three isolates were positive for lipase production and capable of degrading tributyrin. In the secondary screening method, DNT-2 showed high activity with 22 mm of oil spreading activity and 20 mm of drop collapsing activity. The emulsifying capacity of DNT-3, DNT-2 and DNT-5 was evaluated using E24 emulsification index where isolates showed 52%, 86% and 43% of activity respectively. Among all the isolates DNT-2 showed high surfactant production in all screening assays. Potential isolate DNT-2 was identified as Bacillus thuringiensis using biochemical tests and molecular 16s r RNA sequencing analysis..


Cite this article:
Deblina D, Namrata M, Taishee P, Veena S, Bhaskara Rao K.V. Evaluation of Biosurfactant producing activity of Bacillus thuringiensis-VITNDUVNB04 isolated from oil contaminated soil by various methods. Research J. Pharm. and Tech 2016; 9(12):2186-2190. doi: 10.5958/0974-360X.2016.00443.1


Recomonded Articles:

Author(s): Sajid Ali, Maryam Shabbir, Nabeel Shahid

DOI: 10.5958/0974-360X.2015.00019.0         Access: Open Access Read More

Author(s): J. Satyanarayana, A. Pavan Kumar, V. Sai Kishore, T.E. Gopala Krishna Murthy

DOI: Not Available         Access: Open Access Read More

Author(s): Samiksha Warke, Prashant Warke, M.K. Kale, Vipin Saini

DOI: Not Available         Access: Open Access Read More

Author(s): K.V. Swathi

DOI: 10.5958/0974-360X.2016.00239.0         Access: Open Access Read More

Author(s): Srinidhi K R, B.F. Anisha Shabanis, Saipriya G. Shettigar, Venkatesh Kamath B

DOI: 10.5958/0974-360X.2017.00706.5         Access: Open Access Read More

Author(s): Sudha Rathod, Tejaswi R. Kale, Deepali A. Shewale, Praveen N. Lohar

DOI: Not Available         Access: Open Access Read More

Author(s): Madan Ranjit Pusapati, Girijasankar Guntuku, Ankamma Chowdary Yarlagadda, Gollapalli Nagaraju, M. Soumya, T.B.V. Lakshmi

DOI: Not Available         Access: Open Access Read More

Author(s): Daljeet Singh Dhanjal, Chirag Chopra, Prince Anand, Reena Singh Chopra

DOI: 10.5958/0974-360X.2017.00612.6         Access: Open Access Read More

Author(s): Saurabh Bharadwaj, U.V.S. Teotia, Kishan Singh, Rajib Sharma, Yogendra Singh

DOI: Not Available         Access: Open Access Read More

Author(s): Vaishvedhidha M.U, Ezilrani. P, Godwin Christopher. J

DOI: 10.5958/0974-360X.2015.00255.3         Access: Open Access Read More

Author(s): Govindasamy Vinodhini, Madhava Anil Kumar, Saptharishi Balamanikandan, Muthulingam Seenuvasan

DOI: 10.5958/0974-360X.2015.00010.4         Access: Open Access Read More

Author(s): Fatemeh Behoftadeh, Ali Mojtahedi, Elham Hooshmandi

DOI: 10.5958/0974-360X.2018.00787.4         Access: Open Access Read More

Author(s): UK Patil, Ravish Sahu, SK Yadav

DOI:         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags