Author(s): Souvik Paul, Kavitha S, Vimala R

Email(s): vimararagu@gmail.com

DOI: 10.5958/0974-360X.2016.00392.9   

Address: Souvik Paul, Kavitha S, Vimala R
School of Biosciences & Technology, VIT University, Vellore, Tamilnadu, India-632014.
*Corresponding Author

Published In:   Volume - 9,      Issue - 11,     Year - 2016


ABSTRACT:
Actinomycetes represent a ubiquitous group of microbes widely distributed in natural ecosystems around the world. They are economically and biotechnologically valuable prokaryotes able to produce wide range of bioactive secondary metabolites useful in medicines like antibiotics, anticancer drugs, immunosuppressant and enzyme inhibitors. In order to facilitate the discovery of novel bioactive compounds from microorganisms, various techniques for isolation of new actinomycetes strains have been followed. A total of eight actinomycetes were isolated from terrestrial soil by spread plate method on Starch casein agar (SCA). Based on the biochemical test and morphological identification four of them were selected and screened for their antimicrobial activity against E.coli and Staphylococcus aureus by agar well diffusion method. Two isolates R1 and R2 have shown the activity against the pathogens. Those isolates were subjected for optimization of various parameters such as carbon sources, nitrogen sources, temperature, pH and NaCl concentration. The isolates were then subjected to solvent extraction using ethyl acetate. Further, the extracts were tested against biofilm forming bacteria Pseudomonas aeruginosa strain NV2 by Micro titer plate assay (MTP) and well diffusion method.


Cite this article:
Souvik Paul, Kavitha S, Vimala R. Isolation of Actinomycetes and Optimization of Process Parameters for Antimicrobial Activity. Research J. Pharm. and Tech 2016; 9(11): 1913-1921. doi: 10.5958/0974-360X.2016.00392.9


Recomonded Articles:

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): Koushika Das, Pranit Krishna, Avipsha Sarkar, Shanmuga Sundari Ilangovan, Shampa Sen

DOI: 10.5958/0974-360X.2017.00267.0         Access: Open Access Read More

Author(s): Abhirup Dey, Mangala Lakshmi Ragavan, Sanjeeb Kumar Mandal, Nilanjana Das

DOI: 10.5958/0974-360X.2017.00136.6         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): A. Julius, Ramachandran Vedasendiyar, Archana Devakannan, Sujatha Rajaraman, Balamurugan Rangasamy, V. Saravanan

DOI: 10.5958/0974-360X.2017.00062.2         Access: Open Access Read More

Author(s): Dhanalekshmi UnniKrishnan Nair, MP Saraswathy, Narra Kishore, Neelakanta Reddy Pully

DOI: Not Available         Access: Open Access Read More

Author(s): T. Arunkumar, Ann Feba Ebby, G. Narendrakumar

DOI: 10.5958/0974-360X.2017.00441.3         Access: Open Access Read More

Author(s): Amutha K*, Ashok Kumar A, Godavari A

DOI: 10.5958/0974-360X.2017.00100.7         Access: Open Access Read More

Author(s): Krishnendu Acharya*, Somanjana Khatua, Salman Sahid

DOI: Not Available         Access: Open Access Read More

Author(s): Sajid Ali, Maryam Shabbir, Nabeel Shahid

DOI: 10.5958/0974-360X.2015.00019.0         Access: Open Access Read More

Author(s): Pooja Thakur, Chirag Chopra, Daljeet Singh Dhanjal, Reena Singh Chopra

DOI: 10.5958/0974-360X.2017.00466.8         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags