ABSTRACT:
Majority of ESBLs are derived from the amino acid substitutions of their parent enzymes TEM and SHV ß-lactamases, but there are several other types of acquired ESBLs found in Enterobacteriaceae. Among these ESBLs, TEM, SHV and CTX-M types of are of being major clinical concern and predominated in Gram-negative bacteria. In this study we have taken 20 clinical isolates of E.coli and were subjected to antibiotic sensitivity pattern followed by the detection of blaTEM-1gene by PCR. We have observed total resistance to cephalosporins followed by other group of drugs. 20% of our strains were found to have this gene. This indicates that apart from this gene there several other genes that encodes for ESBLs such as CTX-M, SHVetc may be the reason for showing the resistance. To rule out this more number of isolateshave to be included with a battery of other genes to be screened to get the actual outcome.