Alpha hemolysin is a toxin secreted by Escherichia coli causes cell death by binding with the outer membrane, with subsequent oligomerization of the toxin monomer and water-filled channels. These are responsible for osmotic phenomena, cell depolarization, and loss of vital molecules ,leading to its demise. A total of 20 clinical isolates of E. coli were screened for the presence of hlyA gene by PCR. We have observed 60% positivity among our isolates. There are several virulent factors that are associated with urinary tract infections, production of alpha hemolysin one amongst those factors also play a crucial role in pathogenesis of UTI.
Cite this article:
Nirisha Sriram, Gopinath .P. Detection of Hly-A Gene for the production of Alpha Hemolysin in Clinical Isolates of Escherichia coli. Research J. Pharm. and Tech 2016; 9(10):1629-1631. doi: 10.5958/0974-360X.2016.00325.5