Author(s): Samridhi, Sandeep Kumar Singh

Email(s): dr.sandeep_pharmaceutics@yahoo.com

DOI: 10.5958/0974-360X.2015.00076.1   

Address: Samridhi, Sandeep Kumar Singh*
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi- 835215, Jharkhand, India
*Corresponding Author

Published In:   Volume - 8,      Issue - 4,     Year - 2015


ABSTRACT:
The objective of the present work was to develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and UV-spectrophotometric method and to validate the methods as per ICH guidelines for the quantitative estimation of loratadine. The optimized method employed a C18 ODS column (150×4.6mm, 5µm), a mobile phase of 35:45:20 (v/v) mixture of acetonitrile, methanol and a phosphate buffer solution (0.01M, pH 7.2±0.1), flow rate of 1.0 mL/min and a detection wavelength of 245nm (UV detector). A simple, sensitive and reliable UV-spectrophotometric method has also been developed. The proposed methods of loratadine in methanol were found to be precise with retention time (RT) at 3.59 min (0.295% RSD) and absorption maxima at 247.0 nm (0.072% RSD). The optimized methods of loratadine in 0.1 N HCl (dissolution media) was also found to be precise with RT of 3.60 min and absorption maxima at 280.0 nm. Results of the linearity studies were statistically validated and accuracy was established by drug recovery within the acceptable limits of 98-102%. The limits of detection and quantitation were determined for both the analytical systems. These validated methods were employed for the determination of loratadine release from lipidic formulations and the model independent similarity approach was used to compare the dissolution profiles. The results obtained by either of these methods equally resulted in efficient estimation of drug. However, spectrophotometric method can also present a reliable and reasonably accurate alternative for the determination of loratadine in pharmaceutical formulations.


Cite this article:
Samridhi, Sandeep Kumar Singh. Validation of Isocratic RP-HPLC Method and UV Spectrophotometric Method for the Estimation of Loratadine in Pharmaceutical for Mulations. Research J. Pharm. and Tech. 8(4): April, 2015; Page 452-461. doi: 10.5958/0974-360X.2015.00076.1


Recomonded Articles:

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Archana Kushwaha, Jayanti Jaiswal, Priya Singh, Neha Rathore, Jai Prakash Dhruw, Ishu Sahu, Chhaya Singh, Krinsha Kumar Sahu, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00270.0         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Homendra Kumar Sahu, Kamdev Sen, Raju Patel, Hulash Sahu, Deepika Sahu, Gulshan Sahu, Chandrashekhar Jain, Narayan Prasad Chandra, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00173.1         Access: Open Access Read More

Author(s): Hemlata M. Nimje, Rajesh J. Oswal, Sandip S. Kshirsagar, Manoj Chavan

DOI: Not Available         Access: Open Access Read More

Author(s): Vikram Kumar Sahu, Amit Mishra, Nitin Sharma, Pratap Kumar Sahu, Shubhini A. Saraf

DOI: 10.5958/0974-360X.2017.00048.8         Access: Open Access Read More

Author(s): Rao Rekha, Goswami Anju, Shekhar Shishant, Mahant Sheefali, Nanda Sanju

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags