Author(s): Rele Rajan V.

Email(s): drvinraj@gmail.com

DOI: 10.5958/0974-360X.2015.00044.X   

Address: Rele Rajan V.*
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016.
*Corresponding Author

Published In:   Volume - 8,      Issue - 3,     Year - 2015


ABSTRACT:
Simple and precise UV spectrophotometric methods by second order derivative and area under curve [AUC] have been developed and validated for the estimation of olopatadine hydrochloride in bulk and its pharmaceutical formulation. The standard and sample solutions of olopatadine hydrochloride were prepared in methanol. Olopatadine hydrochloride was estimated at 224 nm for the second order derivative UV-spectrophotometric method (A), while in area under curve (AUC) method (B) the zero order spectrum of olopatadine hydrochloride was measured in between 220 nm to 230 nm. Beer’s law was obeyed in the concentration range of 1 to 16 µg / ml with coefficient of correlation value 0.9994 for second order derivative method. Similarly in AUC method, Beer’s law was obeyed in the concentration range of 1 to 12 µg / ml with coefficient of correlation value 0.9991. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were of 0.1311 % and 0.5889 % for the above two methods respectively. The proposed methods were successfully applied for the determination of olopatadine hydrochloride in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.


Cite this article:
Rele Rajan V. UV-Spectrophotometric Estimation of Olopatadine hydrochloride in Bulk and Pharmaceutical Dosage Form by area under Curve and second Order Derivative Methods. Research J. Pharm. and Tech. 8(3): Mar., 2015; Page 265-269. doi: 10.5958/0974-360X.2015.00044.X


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DOI: 10.5958/0974-360X 

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