Author(s): R. Vani, B. Vijaya Kumar, G. Krishna Mohan

Email(s): vrathipelli@gmail.com

DOI: 10.5958/0974-360X.2015.00038.4   

Address: R. Vani1*, B. Vijaya Kumar2, G. Krishna Mohan3
1Research Scholar, JNTU-K, Kakinada and Deccan School of Pharmacy, Hyderabad.
2Principal Jangaon Institute of Pharmaceutical Sciences, Jangaon.
3Center for Pharmaceutical Sciences, JNTU-H Hyderabad.
*Corresponding Author

Published In:   Volume - 8,      Issue - 2,     Year - 2015


ABSTRACT:
A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Allopurinol and Alpha lipoic acid in bulk and pharmaceutical dosage form using C18 column (inertsil ODS, 250 x 4.6 mm, 5 µm) in isocratic mode. The mobile phase consisted of 0.1 M Dipotassium Phosphate buffer (pH 3.5) and acetonitrile in the ratio of 55:45v/v. The detection was carried out at 250 nm for Allopurinol and 212 nm for Alpha lipoic acid. The method was linear over the concentration range for Allopurinol 60-140 µg/ml and for Alpha lipoic acid 60-140µg/ml. The recoveries of Allopurinol and Alpha lipoic acid were found to be 98.9% and 98.7% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.


Cite this article:
R. Vani, B. Vijaya Kumar, G. Krishna Mohan. Analytical Method Development and Validation for the Determination of Allopurinol and Alphalipoic Acid Using Reverse Phase HPLC Method in Bulk and Tablet Dosage Form. Research J. Pharm. and Tech. 8(2): Feb. 2015; Page 207-211. doi: 10.5958/0974-360X.2015.00038.4


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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