Author(s): K. Vijaya Sri, M. Madhuri, G. Vinay Jain and K. Suresh

Email(s): Email ID Not Available

DOI: Not Available

Address: K. Vijaya Sri, M. Madhuri, G. Vinay Jain and K. Suresh
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad-500 014 Andhra Pradesh, India
*Corresponding Author

Published In:   Volume - 7,      Issue - 8,     Year - 2014


ABSTRACT:
A simple, rapid, sensitive, linear, precise, accurate, rugged and robust RP-HPLC-PDA method was developed and validated for the determination of emtricitabine in bulk and capsule dosage form. Reversed-phase chromatography was performed on BDS hypersil C-18 (250 mm × 4.6 mm, 5 micrometer) column of Waters HPLC with Empower software. Methanol: water: acetonitrile 80:13.5:6.5 (v/v/v) was used as mobile phase at a flow rate of 1 mL min-1 with PDA detection at 239 nm. The retention time of emtricitabine was 2.78 min. Linearity was observed in the concentration range of 2-200 microgram mL-1 with regression equation y = 53699x + 122068 (R2 = 0.9998). The method was validated as per ICH guidelines. The %RSD for intra-day and inter-day precision were found to be less than 1 %. The percentage assay of emtricitabine was 100.44% and the percentage recovery was in good agreement with the labelled amount in the pharmaceutical formulation. The proposed method is simple, rapid, sensitive, linear, precise, accurate, robust and rugged and hence applied for the determination of emtricitabine in bulk and pharmaceutical dosage form.


Cite this article:
K. Vijaya Sri, M. Madhuri, G. Vinay Jain and K. Suresh. Rapid RP-HPLC Method for Quantitative Determination of Emtricitabine in Bulk and Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 7(8): August 2014 Page 897-901.


Recomonded Articles:

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Archana Kushwaha, Jayanti Jaiswal, Priya Singh, Neha Rathore, Jai Prakash Dhruw, Ishu Sahu, Chhaya Singh, Krinsha Kumar Sahu, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00270.0         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Homendra Kumar Sahu, Kamdev Sen, Raju Patel, Hulash Sahu, Deepika Sahu, Gulshan Sahu, Chandrashekhar Jain, Narayan Prasad Chandra, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00173.1         Access: Open Access Read More

Author(s): Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj

DOI: 10.5958/0974-360X.2015.00299.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags