Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao
Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao*
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division,
School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India.
Volume - 7,
Issue - 5,
Year - 2014
The aim of the present study was to isolate L-asparaginase producing actinobacteria from mangrove sediment. The samples were collected from Parangapattai coastal area of Tamilnadu, India. A total of 24 actinobacteria colonies were isolated, among them 14 strains were separated and selected based on colony morphology. The isolated actinobacterial strains were screened for L- Asparginase enzyme production on M9 medium. Out of 14 isolates, 4 isolates (VITPPH1, VITPPH6, VITPPH7 and VITPPH10) showed positive results in Rapid plate Assay method. All the four actinobacterial strains showed 25-60 µg ammonia/mL/h range of L-asparagines activity. VITPPH10 strain showed highest L-asparaginase activity (58 µg ammonia/mL/h). Among the 14 isolates, VITPPH10 isolate showed best antimicrobial activity against Salmonella typhi and Pseudomonas aeruginosa. Cytotoxic assay was determined by using brine shrimp assay. VITPPH10 showed 100% mortality within 24 hours. The potential actinobacterial strain VITPPH10 was identified as Streptomyces sp.
Cite this article:
Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao. Production of L-Asparaginase by Marine Actinobacteria Isolated from the Rhizosphere of Mangrove Plants. Research J. Pharm. and Tech. 7(5): May, 2014; Page 526-530.