Author(s): Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao

Email(s): kokatibhaskar@yahoo.co.in

DOI: Not Available

Address: Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao*
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 7,      Issue - 5,     Year - 2014


ABSTRACT:
The aim of the present study was to isolate L-asparaginase producing actinobacteria from mangrove sediment. The samples were collected from Parangapattai coastal area of Tamilnadu, India. A total of 24 actinobacteria colonies were isolated, among them 14 strains were separated and selected based on colony morphology. The isolated actinobacterial strains were screened for L- Asparginase enzyme production on M9 medium. Out of 14 isolates, 4 isolates (VITPPH1, VITPPH6, VITPPH7 and VITPPH10) showed positive results in Rapid plate Assay method. All the four actinobacterial strains showed 25-60 µg ammonia/mL/h range of L-asparagines activity. VITPPH10 strain showed highest L-asparaginase activity (58 µg ammonia/mL/h). Among the 14 isolates, VITPPH10 isolate showed best antimicrobial activity against Salmonella typhi and Pseudomonas aeruginosa. Cytotoxic assay was determined by using brine shrimp assay. VITPPH10 showed 100% mortality within 24 hours. The potential actinobacterial strain VITPPH10 was identified as Streptomyces sp.


Cite this article:
Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao. Production of L-Asparaginase by Marine Actinobacteria Isolated from the Rhizosphere of Mangrove Plants. Research J. Pharm. and Tech. 7(5): May, 2014; Page 526-530.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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