Author(s): S. Shalini Devi, Y. Sreenivasulu, K.V. Bhaskar Rao

Email(s): yasodam.sri@gmail.com

DOI: Not Available

Address: S. Shalini Devi1, Y. Sreenivasulu2* and K.V. Bhaskar Rao1
1School of Biosciences and Technology, VIT University, Vellore 632 014,Tamilnadu, India.
2Watson Life Sciences, Tirupati-517 502, A.P, India.
*Corresponding Author

Published In:   Volume - 7,      Issue - 4,     Year - 2014


ABSTRACT:
Fungi were isolated from Marine water collected from three different regions of Andhra Pradesh, India. A total number of 45 fungi were isolated of which 9 showed varied sized clear zones on assay medium. Largest clear zone was detected below and around the fungal colony WFPHB2, T.verruculosus for which the PHB (Polyhydroxybutyrate) depolymerase productivity has not yet been published. This strain grew well on PDA (Potato Dextrose Agar) medium. In early stage of growth, enzyme production was highest at 30oC. Purification and characterization of PHB depolymerase secreted by WFPHB2 was carried which reported that single protein band of 45 kDa is responsible for depolymerase activity. 80µl of the purified enzyme showed clear degradation zone during plate assay. 96 hrs incubation in liquid broth containing WFPHB2 resulted in 90%depolymerization of PHB films.


Cite this article:
S. Shalini Devi, Y. Sreenivasulu, K.V. Bhaskar Rao. Talaromyces verruculosus, A Novel Marine Fungi as a Potent Polyhydroxybutyrate Degrader. Research J. Pharm. and Tech. 7(4): April, 2014; Page 433-438.


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