Author(s): Prafulla M. Sabale, Jignesh Chavda, Vidya Sabale

Email(s): prafullasable@yahoo.com

DOI: Not Available

Address: Prafulla M. Sabale1*, Jignesh Chavda2, Vidya Sabale3
1Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University,Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur-440 033 India
2Department of Pharmaceutical Quality Assurance, Parul Institute of Pharmacy, Limda-391 760, Vadodara, Gujarat, India
3Department of Pharmaceutics, Parul Institute of Pharmacy and Research, Limda-391 760, Vadodara, Gujarat, India
*Corresponding Author

Published In:   Volume - 7,      Issue - 2,     Year - 2014


ABSTRACT:
The present research work describes the development of a new simple, precise, sensitive and validated RP-HPLC method for Zn-sparfloxacin 1,10-phenanthroline metal complex. The chromatographic conditions used for the separation was Phenomenex Luna C18 (250mm × 4.6mm, 5micrometer particle size) and mobile phase comprised of methanol: phosphate buffer (30:70 v/v) and pH was adjusted to 3 by o-phosphoric acid. The flow rate was 1.0 ml/min with detection at 293 nm. The retention time was found to be 4.357 min. The linearity was found to be in the range of 2-10 micrometer/ml for Zn-spar-phe metal complex with correlation coefficient of 0.9996. The proposed method is accurate with 98-102 % recovery and precise (%RSD of repeatability, intra-day and inter-day variations were 0.240, 0.7989-1.1386 and 1.09-1.53 respectively). The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.2658 and 0.8056 microgram/ml respectively. Proposed method was validated as per ICH guidelines for precision, accuracy and linearity for estimation of Zn-spar-phe Metal complex. Results of the validation were found satisfactory.


Cite this article:
Prafulla M. Sabale, Jignesh Chavda, Vidya Sabale. Development and Validation of RP-HPLC Method for Estimation of Zinc-Sparfloxacin 1,10-Phenanthroline Metal Complex. Research J. Pharm. and Tech. 7(2): Feb. 2014; Page 155-160.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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