Author(s): Rajan V. Rele, Swapnil A. Sawant, Sachin S. Patil

Email(s): drvinraj@gmail.com

DOI: Not Available

Address: Rajan V. Rele*, Swapnil A. Sawant, Sachin S. Patil
Central Research Laboratory, D.G. Ruparel College, Matunga, Mumbai 400016.
*Corresponding Author

Published In:   Volume - 6,      Issue - 8,     Year - 2013


ABSTRACT:
The objective of the study was to develop a simple, accurate, precise and rapid a UV area under curve spectrophotometric method for the determination of ofloxacin and ornidazole by using 0.1N hydrochloric acid as the solvent in combined dosage form. The method was further validated by ICH guidelines. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s rule and Matrix method” .The two analytical wavelengths ranges were used i.e. 288-298 nm and 268.6-285 nm for estimation of ofloxacin and ornidazole. The linearity of the proposed method was found in the concentration range of 0.5 – 10 µg /ml (r2= 0.9999) for ofloxacin and 2.0 – 30 µg /ml (r2=0.9999) for ornidazole respectively. The percentage mean recovery was found to be 100.04% for ofloxacin and 100.11 % for ornidazole respectively. The method was statistically validated for its linearity, accuracy and precision as per ICH guidelines. Both intra and inter day variation showed less percentage (%) RSD values indicating high grade of precision of this method.


Cite this article:
Rajan V. Rele, Swapnil A. Sawant, Sachin S. Patil. Simultaneous Spectrophotometric Estimation of Ofloxacin and Ornidazole by Area Under Curve Method in Combined Dosage Form. Research J. Pharm. and Tech. 6(8): August 2013; Page 894-898.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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