Author(s): Nandini R. Pai, Swapnali Suhas Patil

Email(s): nandini_pai@hotmail.com , swapnali22@gmail.com

DOI: Not Available

Address: Dr. Nandini R. Pai * and Swapnali Suhas Patil
Department of Chemistry, D.G. Ruparel College, Mahim, Mumbai 400016, Maharashtra, INDIA
*Corresponding Author

Published In:   Volume - 6,      Issue - 7,     Year - 2013


ABSTRACT:
Simple and sensitive RP-HPLC methods are described for the determination of Levosalbutamol sulphate and Ipratropium bromide combination. The high-performance liquid chromatographic method was developed for the determination of Levosalbutamol sulphate and Ipratropium bromide Pressurized meter dose inhaler (LI-MDI) using acetonitrile: buffer [30: 70] as the mobile phase and measuring the response at Lambda max 210 nm. The analysis was performed on a Chromatopak C18 (150 X 4.6 mm), 5-micrometer column. The method was found to be accurate, with linearity ranging from (80.0% to 120%) for HPLC with a correlation coefficient (r2) 0.999. Precision mean % assay was found within range.


Cite this article:
Nandini R. Pai, Swapnali Suhas Patil. Development and Validation of RP-HPLC Method for Estimation of Levosalbutamol Sulphate and Ipratropium Bromide in Pressured Meter Dose Inhaler Form. Research J. Pharm. and Tech 6(7): July 2013; Page 774-779.


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