Author(s): Seema Jadhav, Pournima Morey, Manisha Karpe, Vilasrao Kadam

Email(s): smjadhav226@gmail.com

DOI: Not Available

Address: Seema Jadhav*, Pournima Morey, Manisha Karpe, Vilasrao Kadam
Bharati Vidyapeeth’s College of Pharmacy, University of Mumbai, Sector-8, C.B.D., Belapur, Navi Mumbai- 400614, Maharashtra, India
*Corresponding Author

Published In:   Volume - 6,      Issue - 7,     Year - 2013


ABSTRACT:
Atenolol is a hydrophillic Beta-1 cardioselective adrenergic receptor blocker. It is prescribed widely in treatment of various cardiovascular diseases such as hypertension, angina pectoris, arrhythmias and myocardial infarction. A sensitive, simple, precise and accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for the analysis of Atenolol in bulk drug. The HPLC separation achieved on HiQSil C18HS (4.6 mm X 250 mm) at temperature of 25°C employing mobile phase consisting of 20 mM Ammonium Acetate Buffer (pH 5): Acetonitrile (80:20) with flow rate of 0.75 ml/min was used. The UV detection was done at 273 nm. The retention time was found to be 4.606 min. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to Linearity and Range, System suitability, Selectivity, Precision, Accuracy, Detection limit and Quantitation limit. The method was found to be simple, precise and accurate. The calibration curve was found to be linear in the concentration range of 5 – 40 µg/ml with r2 value of 1. The method was accurate (98.02 to 99.80 %), precise (the % Relative Standard Deviations (% RSD) of repeatability and intermediate precision were 0.3508 and 1.2135 respectively). The detection limit and quantitation limit by standard deviation method for Atenolol drug was found to be 0.075 microgram/ml and 0.23 microgram/ml.


Cite this article:
Seema Jadhav, Pournima Morey, Manisha Karpe, Vilasrao Kadam. RP-HPLC Method Development and Validation for Determination of Atenolol in Bulk Drug. Research J. Pharm. and Tech 6(7): July 2013; Page 736-739.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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