ABSTRACT:
The sponge Pseudoceratina purpurea associated bacteria from Pongibalu region of Andaman islands, India and isolate the methanolic extract of P. purpurea for identifying the antibacterial effect against certain pathogenic bacteria. Standard characterization and biochemical methods were employed for identifying the sponge associated bacteria. Antibacterial assay and micro broth dilution test for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were subjected to evaluate the efficacy of P. purpurea extract against several gram positive and gram negative bacteria. Associated bacteria of P. purpurea, 75% were gram negative bacteria except Staphylococcus aureus, Bacillus subtilis and Proteus vulgaris were not susceptible to the sponge extract in well diffusion method. Minimum concentration effect was found in Bacillus thermophilus and Klebsiella pneumoniae against the P. purpurea extract. Overall study apparently showed that the organic extracts of this sponge might be a promising source of secondary metabolites and ascription of toxic metabolites from associated bacteria to find new and useful medicines to treat human and veterinary infections.