Author(s): M.S. Shekhawat

Email(s): smahipal3@gmail.com

DOI: Not Available

Address: M.S. Shekhawat*
Biotechnology Unit, K.M. Centre for Postgraduate Studies, Pondicherry, India
*Corresponding Author

Published In:   Volume - 6,      Issue - 4,     Year - 2013


ABSTRACT:
A highly efficient plant propagation protocol was developed for Euphorbia hirta L. using nodal shoot segments as explant. The explants were surface sterilized with 0.1% (w/v) mercuric chloride on Murashige and Skoog (MS) medium augmented with different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kn) singly or in combination with Indole-3-acetic acid (IAA). Use of BAP at 2.0 mgl-1 induced the highest frequency (100%) of shoot induction as well as maximum number of shoots per explant (6.22). Proliferated shoot clumps were further multiplied and elongated on 0.5 mgl-1 BAP and Kn. The highest rooting frequency (92.8%) as well as highest number of roots (4.3) was observed in half strength MS medium supplemented with 2.0 mgl-1 Indole-3-butyric acid (IBA). Regenerated plantlets were acclimatized successfully in the green house and finally transplanted in the pots.


Cite this article:
M.S. Shekhawat. Direct shoots regeneration from explant surface in Euphorbia hirta L. Research J. Pharm. and Tech. 6(4): April 2013; Page 435-439.


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DOI: 10.5958/0974-360X 

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