Pramod Kumar, Babulal Saini, Ravi Dahiya, Jawed Akhtar, Birendra Shrivastav
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Pramod Kumar1* Babulal Saini1, Ravi Dahiya1, Jawed Akhtar2, Birendra Shrivastav1
1Department of Quality Assurance, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur (Rajasthan) India-302025
2Department of Quality Assurance, Parul Institute of Pharmacy & Research, Baroda
Volume - 6,
Issue - 2,
Year - 2013
A simple, efficient, precise and accurate Vierodt’s method have been developed for the estimation of Hydrochlorothiazide and Lisinopril in pure and in fixed dose Combination. In this method UV spectrum of Hydrochlorothiazide and Lisinopril were overlained which involves the formation of Simultaneous equation at maximum wavelength 274.5 nm and 215 nm. Both the drugs obeyed Beer’s law in the concentration range 5-20 microgram/ml and 1-10 microgram/ml for Hydrochlorothiazide and Lisinopril, respectively. The accuracy of the method was determined by recovery studies and was found to be 100.04±0.638% and 99.13±0.404% for Hydrochlorothiazide and Lisinopril, respectively in Synthetic Mixture. The method was validated as per ICH guidelines and statically. The method showed good reproducibility and recovery with % RSD less than 2. The method was found to be simple, economic, accurate and reproducible and can be used for routine analysis of Hydrochlorothiazide and Lisinopril in pure and in fixed dose combination.
Cite this article:
Pramod Kumar, Babulal Saini, Ravi Dahiya, Jawed Akhtar, Birendra Shrivastav. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Hydrochlorothiazide and Lisinopril in Bulk Drug and Its Formulations. Research J. Pharm. and Tech. 6(2): Feb. 2013; Page 212-215.