Author(s): Thorat Shashikant D., Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R.

Email(s): thorat_shashikant@ymail.com

DOI: Not Available

Address: Thorat Shashikant D.*, Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R.
Department Pharmaceutical Chemistry, Amrutvahini Collage of Pharmacy, Sangamner, Dist- Ahmednagar, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 6,      Issue - 10,     Year - 2013


ABSTRACT:
A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatography method was developed for simultaneous estimation of Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in powder dosage form on RP C-18 Column (EC 250/4 neucleosil 100-10) using Methanol : Water (97:3 %v/v) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 274nm. The retention time for Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) was found to be as 8.78 and 12.80 respectively. Proposed method was validated for Specificity, Precision, Accuracy, Linearity, and Robustness.


Cite this article:
Thorat Shashikant D., Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cholecalcererol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in Powder Dosage Form. Research J. Pharm. and Tech. 6(10): October 2013; Page 1107-1110.


Recomonded Articles:

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): YK Naidu, Raghunadha Reddy S, Koteswara Rao Divi, MP Kalyan Reddy, I Sarath Chandiran, KN Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj

DOI: 10.5958/0974-360X.2015.00299.1         Access: Open Access Read More

Author(s): Oruganti Sai Koushik, V. Himaja, P. Srinivasa Babu, Ramadoss Karthikeyan

DOI: 10.5958/0974-360X.2015.00298.X         Access: Open Access Read More

Author(s): Mahadeva Rao U.S., Suganya M. Utharkar, C. Shanmuga Sundaram

DOI: 10.5958/0974-360X.2015.00307.8         Access: Open Access Read More

Author(s): Rituraj Singh Chundawat, Y.S. Sarangdevot, R.P.S. Rathore, Dharmendra Singh Sisodiya, Udaibhan Singh Rathore

DOI:         Access: Open Access Read More

Author(s): R.V Rele, J.M. Parab, V.V. Mhatre, C.B. Warkar

DOI: Not Available         Access: Open Access Read More

Author(s): Sahiti K, Raji P, Bennett Rohan, Divya Kumar M, Antony V. Samrot

DOI: 10.5958/0974-360X.2016.00064.0         Access: Open Access Read More

Author(s): Poonam P. Patil, Mahesh M. Deshpande, Veena S. Kasture, Seema A. Gosavi

DOI:         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags