Author(s): A.S. Manjula Devi, T.K. Ravi

Email(s): hari1509@gmail.com

DOI: Not Available

Address: A.S. Manjula Devi* and T.K. Ravi
Department of Pharma. Analysis, College of Pharmacy, SRIPMS, Coimbatore, Tamil Nadu, India. PIN 641 044
*Corresponding Author

Published In:   Volume - 5,      Issue - 3,     Year - 2012


ABSTRACT:
A high-performance liquid chromatographic method with PDA detection and UV spectrophotometric method were developed for the quantitative determination of zotepine. Chromatographic separation was achieved on a LiChrospher® RP-18 HPLC column (5 micro particle size and 25 cm × 4.6 mm using 0.5%trifluoroacetic acid:methanol:acetonitrile (5:40:55 v/v/v; pH 2.23) as mobile phase and aceclofenac as the internal standard. The effluent was monitored at 265 nm. Two sharp peaks were obtained for internal standard and zotepine at 3.4 and 5.1min, respectively. UV spectrophotometric method was performed at 265 nm using methanol as the solvent. Analytical performance parameters such as linearity, specificity, accuracy, precision, limit of detection and limit of quantification were determined according to International Conference on Harmonization (Q2B) guidelines. Linear range obtained was 1-10 µg mL-1 (r2>0.99) for HPLC method and 2.5-25 Microgram mL -1 (r2>0.99) for UV spectrophotometric method. Validation as per ICH guidelines and statistical analysis showed that both the methods were precise, accurate, sensitive, and can be used for the routine quality control of zotepine.


Cite this article:
A.S. Manjula Devi, T.K. Ravi. Validated UV spectrophotometric and HPLC methods for quantitative determination of zotepine. Research J. Pharm. and Tech. 5(3): March 2012; Page 342-345.

Cite(Electronic):
A.S. Manjula Devi, T.K. Ravi. Validated UV spectrophotometric and HPLC methods for quantitative determination of zotepine. Research J. Pharm. and Tech. 5(3): March 2012; Page 342-345.   Available on: https://rjptonline.org/AbstractView.aspx?PID=2012-5-3-30


Recomonded Articles:

Author(s): Vishal Vilas Shah, Nutan Dhanpal Shah, Prasad Vasantrao Patrekar

DOI: Not Available         Access: Open Access Read More

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): Karishma Ravinthar, Jayalakshmi

DOI: 10.5958/0974-360X.2018.00148.8         Access: Open Access Read More

Author(s): R. Narayana Charyulu, P. Parvathy Devi, Jobin Jose, A. Veena Shetty

DOI: Not Available         Access: Open Access Read More

Author(s): Rakhi Mehra, Renu Makhija, Neera Vyas

DOI: Not Available         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): G. Bhavani, R. Navaneethan

DOI: 10.5958/0974-360X.2017.00269.4         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Ankit Patel, Pankaj Kushwah, Sujit Pillai, Ajay Raghuvanshi, Nitin Deshmukh

DOI: 10.5958/0974-360X.2017.00013.0         Access: Open Access Read More

Author(s): M. A. Fazeelath Banu, Karthikeyan Murthykumar, Dr. Dhanraj

DOI: 10.5958/0974-360X.2016.00184.0         Access: Open Access Read More

Author(s): Kalpana Kamnoore, M P Venkatesh, Balamuralidhara V, T M Pramod Kumar

DOI: 10.5958/0974-360X.2020.00276.0         Access: Open Access Read More

Author(s): Peethala Prathyusha, Raja Sundararajan, Palyam Bhanu, Mathrusri Annapurna Mukthinuthalapati

DOI: 10.5958/0974-360X.2020.00507.7         Access: Open Access Read More

Author(s): S. Subasree, Karthikeyan Murthykumar, Dhanraj

DOI: 10.5958/0974-360X.2016.00116.5         Access: Open Access Read More

Author(s): Rahar Sandeep, Nagpal Navneet, Swami Gaurav, Arora Manisha, Bansal Suraj, Goyal Sandeep, Singla Shwali, Singh Preeti, Kapoor Reni

DOI: Not Available         Access: Open Access Read More

Author(s): Vijayalakshmi. S , Disalva. X, Chittaranjan Srivastava, Arun A

DOI: 10.5958/0974-360X.2019.00520.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags