Author(s): P. Thulasamma, P. Venkateswarlu

Email(s): ponneri.venkat@rediffmail.com

DOI: Not Available

Address: P. Thulasamma and P. Venkateswarlu*
Department of Chemistry, S.V. University, Tirupati
*Corresponding author

Published In:   Volume - 5,      Issue - 3,     Year - 2012


ABSTRACT:
Nifedipine is antihypertensive and diuretic drug prescribed for angina pectoris, arterial hypertension and Reynaud’s phenomenon. This article deals with a simple, feasible and sensitive isocratic Reverse – phase high performance liquid chromatographic method for the determination of nifedipine in bulk drug, pharmaceutical dosage form, in human milk and urine. Chromatographic separation was conducted on prepacked purospher star, C18 (5 µm, 250 x 4.6 mm) column at room temperature using phosphate buffer: acetonitrile (60:40 v/v) as a mobile phase, pH adjusted at 3.0 with orthophosphoric acid at 242 nm. Validation parameters include linearity, accuracy, precision, specificity, limit of detection (LOD), limit of quantification (LOQ) and robustness over a linearity range 2 – 12 µg ml-1 according to the ICH guidelines (r 0.9991), the inter – day and intra – day precision and relative standard deviation (RSD) 1.28 %. The system suitability was scrutinized by capacity factor, tailing factor, number of theoretical plates and retention time. The established HPLC method is rapid, accurate and selective, because of its sensitivity and reproducibility.


Cite this article:
P. Thulasamma, P. Venkateswarlu. RP-HPLC method for the quantitative determination of nifedipine in dosage forms, human milk and urine. Research J. Pharm. and Tech. 5(3): Mar.2012; Page 322-327.


Recomonded Articles:

Author(s): Ravindranath S. Misal, Vishawas R. Potphode, Vijay R. Mahajan

DOI: 10.5958/0974-360X.2017.00218.9         Access: Open Access Read More

Author(s): Niharika, Navneet Verma

DOI: 10.5958/0974-360X.2016.00182.7         Access: Open Access Read More

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Leelavathi D Acharya, N R Rau, N Udupa, Surulivel Rajan M, Vijayanarayana K

DOI: 10.5958/0974-360X.2016.00162.1         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Archana Kushwaha, Jayanti Jaiswal, Priya Singh, Neha Rathore, Jai Prakash Dhruw, Ishu Sahu, Chhaya Singh, Krinsha Kumar Sahu, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00270.0         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Homendra Kumar Sahu, Kamdev Sen, Raju Patel, Hulash Sahu, Deepika Sahu, Gulshan Sahu, Chandrashekhar Jain, Narayan Prasad Chandra, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00173.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags