ABSTRACT:
Tolnaftate (O-2-Naphthyl m, N-dimethylthiocarbanilate) is a topically used antifungal agent, most commonly employed in the treatment of tinea pedis at 1% (w/w) concentration. Analysis of this thionocarbamate fungicide which is also antidermatophytic, antitrichophytic and antimycotic agent primarily inhibits the ergosterol biosynthesis in the fungus. The spectra of Tolnaftate in methanol showed maximum wave length at 257nm and obeyed Beer’s law in the concentration range of 1-5 microgram/ml. Standard curve depict line equation y =0.148x-0.024 with correlation coefficient of 0.990. The developed method was validated with respect to linearity. The objective of present work is to develop simple, precise and accurate UV Spectrophotometric method for estimation of Tolnaftate in organic solvent. The organic solvent methanol is used was HPLC grade. This method can also be used for Tolnaftate determination in its marketed formulations.