Nurul Huda Md. Masu, Kaiser Hamid, Abu Hasanat Zulfiker, Kamal Hossain, Kaniz Fatima Urmi
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Nurul Huda Md. Masum1, Kaiser Hamid2*, Abu Hasanat Md. Zulfiker3, Md. Kamal Hossain4, Kaniz Fatima Urmi5
1Department of Pharmacy, Southeast University, Bangladesh
2*Lecturer, Department of Pharmacy, East West University, Bangladesh
3Department of Pharmacy, Southeast University, Bangladesh
4Vetafarm Manufacturing Pty. Ltd, Wagga Wagga, NSW, Australia
5Department of Pharmacy, Jahangirnagar University, Bangladesh
Volume - 5,
Issue - 12,
Year - 2012
The aim of the present study was to evaluate and compare the in vitro antioxidant activity of different parts of the plant Moringa oleifera lam. Antioxidant activity was measured based on the DPPH radical scavenging assay, Nitric oxide scavenging assay, determination of total phenol, total flavonoids, total antioxidant content and reducing power assay. Ethyl acetate fraction of both bark and leaves showed potent free radical scavenging activity with IC50 value of 14.47 and 29.91 (µg/ml) respectively. The IC50 value of standard (Ascorbic acid) was 33.77 (µg/ml). It was observed that ethyl acetate fraction of fruit contain highest amount of phenolics (613.023±4.11108) expressed as mg Gallic acid equivalents (GAE)/gm of plant extract. In case of total flavonoid content, pet ether fraction of bark contains 2453.1±16.4443 (expressed as mg of quercetin equivalents/gm of plant extract). All fractions contains significant amount of ascorbic acid equivalents ranging from 445.581±0.8222-1162.44±82.222 as mg of ascorbic acid equivalents per gram of plant extract. In case of nitric oxide scavenging assay, ethyl acetate and chloroform fractions of bark and fruit of Moringa oleifera showed potential antioxidant effect having IC50 values ranging from 2.9-87.83 µg/ml. For reducing power assay, all extracts showed increase in the absorbance with increase in concentration. The present study supplements and at the same time compared the presence of antioxidant compounds in different parts of the plant M. oleifera. The next step is to identify the individual compound and their role in different chronic diseases.
Cite this article:
Nurul Huda Md. Masu, Kaiser Hamid, Abu Hasanat Zulfiker, Kamal Hossain, Kaniz Fatima Urmi. In vitro Antioxidant Activities of Different parts of the Plant Moringa oleifera Lam. Research J. Pharm. and Tech. 5(12): Dec. 2012; Page 1532-1537.
Nurul Huda Md. Masu, Kaiser Hamid, Abu Hasanat Zulfiker, Kamal Hossain, Kaniz Fatima Urmi. In vitro Antioxidant Activities of Different parts of the Plant Moringa oleifera Lam. Research J. Pharm. and Tech. 5(12): Dec. 2012; Page 1532-1537. Available on: https://rjptonline.org/AbstractView.aspx?PID=2012-5-12-3