In present study in vitro anticancer activity of ethanolic and aqueous extracts of leaves and stems of Peristrophe bivalvis Merrill was evaluated. In vitro anticancer activity was assayed by tryphan blue dye exclusion method and brine shrimp lethality bioassay method. Extracts were found to be cytotoxic in dose dependent manner in both methods. Ethanolic extract was found to be potent than aqueous extract. Leaves showed more significant anticancer activity as compared to stems. The CTC50 of ethanolic extract of leaves was found to be 175.25±12.34 µg/ml in EAC cell lines. The LC50 of ethanolic extract of leaves was176.49±7.43 µg/ml. It indicated that Peristrophe bivalvis Merrill has potential to prevent cancer in a dose dependent manner.
Cite this article:
Sangeeta S. Tanavade, Smt. Nilofer Naikwade, Dhanyakumar D. Chougule. In vitro anticancer activity of Ethanolic and Aqueous Extracts of Peristrophe bivalvis Merrill. Research J. Pharm. and Tech. 5(10): October 2012; Page 1324-1327.
Sangeeta S. Tanavade, Smt. Nilofer Naikwade, Dhanyakumar D. Chougule. In vitro anticancer activity of Ethanolic and Aqueous Extracts of Peristrophe bivalvis Merrill. Research J. Pharm. and Tech. 5(10): October 2012; Page 1324-1327. Available on: https://rjptonline.org/AbstractView.aspx?PID=2012-5-10-9
society (2006). A biotechnological company dedicated to cancer
DA., Lemke TL., Foye’s principles of Medicinal Chemistry’ 5th
edition, Wolters Kluwer Health (P)Ltd., 2002, pp.462-466,761-782.
Wealth of India- A dictionary of Indian raw materials and Industrial products,
Vol.VII, N-Pe, CSIR, New Delhi,1992, pp.313-314.
RK., Chourasia L. and Katiyar S., Potential antifungal plants for controlling
building fungi, Natural Product Radiance,
7(4), 2008 :374-387.
Purohit AP., Gokhale SB., ‘Pharmacognosy’, Nirali Prakashan, 2006, pp. 111-113.
Randive KJ., Anticancer screening of SAN-AB, An extract of marking nut Semicarpus anacardium. Indian Journal of
Experimental Biology, 9,
Karagoz ID., et.al, Ehrlich ascites carcinoma, African Journal of Biotechnology, 10(13), 2011:2375-2378.
Choudhary MI., and Thomsen WJ., Bioassay Techniques for drug development Taylor
and Francis group, New York, 2000, pp. 9 -12:14 -27,28 -39.
Subhadradevi V., Christy
J., Asok Kumar K., Umamaheshwari and M., Sivashanmugham AT., Induction of
apoptosis and cytotoxic activities of methanolic extract of Careya arborea Roxb bark, Pharmacie Globale (International Journal of
Comprehensive Pharmacy), 01( 3) 2010:1 -3.
Gupta M., Mazumdar
UK., Haldar PK., et. al, Anticancer activity of Indigofera aspalathoides and Wedelia
calendulaceae in swiss albino mice, Iranian
Journal of Pharmacy Research,
Apu AS. Muhit MA.
Tareq SM., Pathan AH.et al, Antimicrobial activity and brine shrimp lethality
bioassay of the leaves extract of Dillenia
indica linn, Journal of Young Pharmacy,
2,2008: 50 -54.
Agrawal SS., Saraswati S., Mathur R. Pandey
M., Cytotoxic and antitumor effects of brucine on Ehrlich ascites tumor and
human cancer cell line. Life Sciences,
Janardhanan KK., Cytotoxic and antitumor activities of a polypore macrofungus, Phellinus rimosus (Berk) pilat, Journal of Ethnopharmacology, 84, 2003
16. Bala A., Kar B.,
Haldar PK., Mazumder UK., Bera S., Evaluation of anticancer activity of cleome gynandra on Ehrlich’s Ascites
Carcinoma treated mice, Journal of Ethnopharmacology,129, 2010:131-143.