Author(s): T. Nageswara Rao1*, G. Silpa2 and T. Srinivasarao3

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Address: 1Department of Engg Chemistry, Andhra University, Visakhapatnam, AP, India 2Department of Biotechnology, Indian Institute of Technology (IIT), Madras, TamilNadu, India 3Johnson& Johnson-Health Care Products & Pharmaceuticals, Mumbai, Maharashtra, India.

Published In:   Volume - 5,      Issue - 10,     Year - 2012


ABSTRACT:
The aim of the present work was to develop and validate a liquid chromatographic method for the determination of memantine hydrochloride in tablet dosage form. Chromatographic separation was achieved on a intersil ODS 3v column (150×4.6 mm), 5 µm column with a HPLC grade methanol and 0.1% trifluroacetic acid buffer in the ratio of 50:50 (v/v/v) as mobile phase under ELS Detector conditions like a evaporation temperature 700 C, nebulization temperature 400 C, nitrogen flow rate 1.2SLM, and LED intensity 90%, injection volume is 20µL, flow rate is 1.0 mL/min and the expected retention of memantine hydrochloride peak is about 6.8 minutes. Response was a linear function of concentration in the range 2-0.01 µg/mL for memantine hydrochloride; correlation coefficiencent was 0.9998, respectively. LOD and LOQ for memantine hydrochloride were found 0.01 µg/mL and 0.03 µg/mL based on signal to noise ratio(S/N). Accuracy (recoveries 88-96%) and reproducibility were found to satisfactory. The present method can be applied successfully for the quantification of active pharmaceutical ingredient content in the combined formulations of memantine hydrochloride.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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