The plant Cuscuta reflexa Roxb. belonging to family Convolvulaceae was selected for the project, on the basis of ethnobotanical information and easy availability. The whole plant of Cuscuta reflexa Roxb. were successively extracted with Petroleum ether and Ethyl acetate by soxhlet extraction. Preliminary Phytochemical screening of Ethyl acetate extract of whole plant of Cuscuta reflexa Roxb. resulted in the identification of various chemical constituents such as - Cardiac glycosides, Phenols and Tannins. Further the Ethyl acetate extract were vacuum dried and subjected for Antibacterial activity by Agar well diffusion method. Zone of inhibition produced by Ethyl acetate extract in dose 25, 50, 100, 150mg/ml against human pathogenic bacteria stains gram positive and gram negative respectively Mycobacterium tuberculosis (MTCC 300) and Salmonella typhimurium (MTCC 98) were measured and compared with standard antibiotics Ciprofloxacin (10µg/ml). The present study demonstrated that the Ethyl acetate extract of Cuscuta reflexa Roxb. shows potent Antibacterial activity against MTCC 98 and MTCC 300 at concentration 150mg/ml respectively. So on the basis of the zone of inhibition we are concluded that Ethyl acetate extract shows more potent Antibacterial activity against gram negative Salmonella typhimurium (MTCC 98) bacterial stains and studies revealed that the Ethyl acetate extract of Cuscuta reflexa Roxb. having Antibacterial activity.
Cite this article:
Manore D., Pillai S., Joshi A., Punashiya R. Preliminary Phytochemical Screening and Antibacterial Activity of Ethyl Acetate Extract of Cuscuta reflexa Roxb. Research J. Pharm. and Tech. 5(1): Jan. 2012; Page 79-82.