Author(s): Halde Supriya, Mungantiwar Ashish, Chintamaneni Meena

Email(s): supriyagujar@hotmail.com

DOI: Not Available

Address: Halde Supriya1,2*, Mungantiwar Ashish1 and Chintamaneni Meena2
1Macleods Pharmaceuticals Ltd., G-2, Mahakali Caves Road, Shanti Nagar, Andheri West, Mumbai-400093
2School of Pharmacy and Technology Management, Narsee Monjee Institute of Management and Higher Studies University, Vile Parle (West), Mumbai-400 056
Corresponding author

Published In:   Volume - 5,      Issue - 1,     Year - 2012


ABSTRACT:
Emtricitabine is a nucleoside agent that has activity against both human immunodeficiency virus and hepatitis B virus. There are very few methods reported in the literature for estimation of emtricitabine using liquid chromatographic with mass spectrometer. The aim of the experiment was to develop a selective, sensitive, rugged and high throughput high performance liquid chromatography tandem mass spectrometric method for the estimation of Emtricitabine from human plasma. Emtricitabine was extracted from human plasma by solid phase extraction using Water Oasis HLB cartridges. The samples were chromatographed on Hypurity Advance, 50 x 2.1 mm, 5µ column using a mobile phase consisting of 5 mM ammonium acetate: Acetonitrile (30:70 v/v). The chromatographic separation is achieved in 1.2 minutes. The method was validated over a concentration range of 50.07 ng/mL to 5006.59 ng/mL. Method was validated for its sensitivity, selectivity, accuracy, precision, matrix effect, recovery and various stabilities. The validated method was used for analysis of plasma samples of bioequivalence study.


Cite this article:
Halde Supriya, Mungantiwar Ashish, Chintamaneni Meena. Determination of Emtricitabine in Human Plasma by LC-MS/MS. Research J. Pharm. and Tech. 5(1): Jan. 2012; Page 133-137.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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