Author(s): Meera Sumanth, Swetha S., Narasimharaju K., Anusha, Natesh T.S.

Email(s): meerasumanth@gmail.com

DOI: Not Available

Address: Meera Sumanth1, Swetha S.1, Narasimharaju K.2, Anusha1 and Natesh T.S.1
1Department of Pharmacology, Visveswarapura Institute of Pharmaceutical Sciences, 22nd main, 24th cross, Banashankari IInd Stage, Bangalore, 560 070 Karnataka, INDIA
2Research Associate, Syngene Intl Ltd, Plot no: 2 and 3, Bommasandra Industrial area, Bangalore 560 100
*Corresponding Author

Published In:   Volume - 4,      Issue - 8,     Year - 2011


ABSTRACT:
Present study was taken up to evaluate genotoxicity of Lipovedic- a polyherbal formulation, by mouse bone marrow micronucleus test (MNT) and chromosomal aberration test (CAT). Since Lipovedic is a drug intended for lifelong use in treatment of hypercholesterolemia it is necessary to evaluate genotoxicity of the drug. Cyclophosphamide (100 mg/kg, i.p) was used as a genotoxic challenge and bone marrow of control and Lipovedic (260mg/kg p.o.× 7 days) treated mice was collected after 24, 48 and 72 h, respectively, after clastogenic challenge. In MNT, the bone marrow smears were stained with May-Grunwald’s followed by Giemsa stain. Polychromatic and Normochromatic erythrocytes were counted and P/N ratio was calculated. Number of micro nucleated PCE and NCE were counted per 2000 erythrocytes. In CAT, Colchicine, 4mg/kg, i.p, was administered 90 min before sacrifice. Bone marrow was collected and smears were prepared, stained with Giemsa stain and observed under 100X for different types of chromosomal aberrations (rings, exchanges, breaks and minutes). Mitotic index was calculated. Lipovedic did not show any mutagenicity, but there was no antimutagenic effect against cyclophosphamide induced clastogenicity. Similarly, chromosomal aberration assay did not reveal any significant alterations. In conclusion, Lipovedic is does not provide protection against mutagenicity, but it is free of genotoxicity. Hence Lipovedic can be used for a long time without any alterations in the genetic apparatus.


Cite this article:
Meera Sumanth, Swetha S., Narasimharaju K., Anusha, Natesh T.S. Genotoxicity Testing of Lipovedic- A Polyherbal Anti-hypercholesterolemic Drug. Research J. Pharm. and Tech. 4(8): Aug. 2011; Page 1189-1192.


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RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

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